Summary of study ST001260

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000846. The data can be accessed directly via it's Project DOI: 10.21228/M8B97R This work is supported by NIH grant, U2C- DK119886.

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Study IDST001260
Study TitleMetabolic changes of Fusobacterium nucleatum when co-cultured with other oral microbes (part-I)
Study SummaryWe used membrane-separated co-culture systems to globally assess metabolomic changes of Fusobacterium nucleatum when co-cultured with Streptococcus gordonii and/or Veillonella parvula.
Institute
Osaka University
DepartmentGraduate School of Dentistry, Department of Preventive Dentistry
Last NameKuboniwa
First NameMasae
AddressYamadaoka 1-8
Emailkuboniwa@dent.osaka-u.ac.jp
Phone81668792922
Submit Date2019-09-26
Analysis Type DetailLC-MS
Release Date2021-04-01
Release Version1
Masae Kuboniwa Masae Kuboniwa
https://dx.doi.org/10.21228/M8B97R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002090 AN002091
Analysis type MS MS
Chromatography type CE CE
Chromatography system Agilent 6210 Agilent 6210
Column None None
MS Type ESI ESI
MS instrument type CE-TOF CE-TOF
MS instrument name Agilent 6210 TOF Agilent 6210 TOF
Ion Mode POSITIVE NEGATIVE
Units AU AU

MS:

MS ID:MS001941
Analysis ID:AN002090
Instrument Name:Agilent 6210 TOF
Instrument Type:CE-TOF
MS Type:ESI
MS Comments:The conditions for measurement of cationic metabolites were as follows. Run buffer: Cation Buffer Solution (H3301-1001; Human Metabolome Technologies (HMT)), CE voltage: +27kV, MS ionization: ESI positive, MS capillary voltage: 4,000V, MS scan range: m/z 50-1,000, and sheath liquid: HMT Sheath Liquid (H3301-1020). Identification of metabolites and evaluation of the relative amounts were conducted using Master Hands (version 2.16.0.15 and 2.17.1.11; Keio University, Tokyo, Japan) with the HMT metabolite database. The relative amount of each metabolite was calculated with reference to the internal standard material (HMT).
Ion Mode:POSITIVE
  
MS ID:MS001942
Analysis ID:AN002091
Instrument Name:Agilent 6210 TOF
Instrument Type:CE-TOF
MS Type:ESI
MS Comments:The conditions for measurement of anionic metabolites were as follows. Run buffer: Anion Buffer Solution (H3302-1023), CE voltage: +30kV, MS ionization: ESI negative, MS capillary voltage: 3,500V, MS scan range: m/z 50-1,000, and sheath liquid: HMT Sheath Liquid (H3301-1020). Identification of metabolites and evaluation of the relative amounts were conducted using Master Hands (version 2.16.0.15 and 2.17.1.11; Keio University, Tokyo, Japan) with the HMT metabolite database. The relative amount of each metabolite was calculated with reference to the internal standard material (HMT).
Ion Mode:NEGATIVE
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