Summary of Study ST002436
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001568. The data can be accessed directly via it's Project DOI: 10.21228/M8142B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002436 |
Study Title | Discovery of phytochelatins in human urine: Evidence for function in selenium disposition and protection against cadmium |
Study Summary | The goal of this project was to detect phytochelatins, plant-derived peptides which function as metal chelators, in human urine. Untargeted metabolomics of 143 urine samples from healthy adults was performed. Phytochelatin 2, γE-C-γE-C-G, was detected, and the rest of the urine metabolome was searched for phytochelatins and predicted phytochelatin metabolites which correlated with phytochelatin 2 concentrations. Phytochelatin 2 and associated metabolites were found to correlate with urinary metals, and further experiments were performed provide insight into function of dietary phytochelatins. |
Institute | Emory University |
Last Name | Jarrell |
First Name | Zachery |
Address | 615 Michael St |
zjarrel@emory.edu | |
Phone | 4047275984 |
Submit Date | 2023-01-10 |
Total Subjects | 143 |
Num Males | 37 |
Num Females | 106 |
Analysis Type Detail | LC-MS |
Release Date | 2023-01-25 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002531 |
Sampleprep Summary: | Samples were prepared following the Emory University Clinical Biomarkers Laboratory SOP for high resolution metabolomics sample preparation. Urine was thawed from -80 °C on ice and vortexed to homogenize. 50 μL urine was transferred to a clean microcentrifuge tube and treated with with 100 μL LC-MS grade acetonitrile containing 2.5 μL internal standard solution. Urine was equilibrated on ice for 30 min. Precipitated proteins were removed by centrifuge (16100 x g at 4 °C for 10 min). 100 μL supernatant was removed and added to an autosampler vial. Samples were maintained at 4 °C until analysis. Samples were prepared alongside NIST SRM 1950 and q3June2014 reference standards. |
Sampleprep Protocol Filename: | EmoryUniversity_HRM_QEHF-MassSpec_092017_v1.pdf |