Summary of Study ST002436
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001568. The data can be accessed directly via it's Project DOI: 10.21228/M8142B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002436 |
| Study Title | Discovery of phytochelatins in human urine: Evidence for function in selenium disposition and protection against cadmium |
| Study Summary | The goal of this project was to detect phytochelatins, plant-derived peptides which function as metal chelators, in human urine. Untargeted metabolomics of 143 urine samples from healthy adults was performed. Phytochelatin 2, γE-C-γE-C-G, was detected, and the rest of the urine metabolome was searched for phytochelatins and predicted phytochelatin metabolites which correlated with phytochelatin 2 concentrations. Phytochelatin 2 and associated metabolites were found to correlate with urinary metals, and further experiments were performed provide insight into function of dietary phytochelatins. |
| Institute | Emory University |
| Last Name | Jarrell |
| First Name | Zachery |
| Address | 615 Michael St |
| zjarrel@emory.edu | |
| Phone | 4047275984 |
| Submit Date | 2023-01-10 |
| Total Subjects | 143 |
| Num Males | 37 |
| Num Females | 106 |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-01-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003969 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters XBridge BEH Amide (50 x 2.1mm,2.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
MS:
| MS ID: | MS003703 |
| Analysis ID: | AN003969 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Raw analysis files from urine analyses were converted to .mzXML using ProteoWizard v3. Data extraction was performed using apLCMS v6.3.3 and xMSanalyzer v2.0.8, generating m/z features consisting of an m/z, retention time (RT) and ion intensity. Feature and sample filtering retained features with a median CV of 50% or less, with a minimum mean Pearson correlation coefficient of 0.7 between technical replicates, and which were detected in at least 30% of samples. Technical replicates were median summarized. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | EmoryUniversity_HRM_QEHF-MassSpec_092017_v1.pdf |
