Summary of Study ST002436
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001568. The data can be accessed directly via it's Project DOI: 10.21228/M8142B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002436 |
Study Title | Discovery of phytochelatins in human urine: Evidence for function in selenium disposition and protection against cadmium |
Study Summary | The goal of this project was to detect phytochelatins, plant-derived peptides which function as metal chelators, in human urine. Untargeted metabolomics of 143 urine samples from healthy adults was performed. Phytochelatin 2, γE-C-γE-C-G, was detected, and the rest of the urine metabolome was searched for phytochelatins and predicted phytochelatin metabolites which correlated with phytochelatin 2 concentrations. Phytochelatin 2 and associated metabolites were found to correlate with urinary metals, and further experiments were performed provide insight into function of dietary phytochelatins. |
Institute | Emory University |
Last Name | Jarrell |
First Name | Zachery |
Address | 615 Michael St |
zjarrel@emory.edu | |
Phone | 4047275984 |
Submit Date | 2023-01-10 |
Total Subjects | 143 |
Num Males | 37 |
Num Females | 106 |
Analysis Type Detail | LC-MS |
Release Date | 2023-01-25 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003969 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters XBridge BEH Amide (50 x 2.1mm,2.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH002935 |
Methods Filename: | EmoryUniversity_HRM-QEHF_chromatography_5min_092017_v1.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters XBridge BEH Amide (50 x 2.1mm,2.5um) |
Column Temperature: | 60 |
Flow Gradient: | A= water, B= acetontrile, C= 2% formic acid in water; 22.5% A, 75% B, 2.5% C hold for 1.5 min, linear gradient to 77.5% A, 20% B, 2.5% C at 4 min, hold for 1 min |
Flow Rate: | 0.35 mL/min for 1.5 min; linear increase to 0.4 mL/min at 4 min, hold for 1 min |
Solvent A: | 100% water |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |