Summary of Study ST002189
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001395. The data can be accessed directly via it's Project DOI: 10.21228/M8DB02 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002189 |
Study Title | Metabolomic profiling reveals muscle metabolic changes following iliac arteriovenous fistula creation in mice (Aqueous) |
Study Type | Study of the skeletal muscle metabolome in mice with iliac arteriovenous fistula via 1H NMR |
Study Summary | In the present study, we hypothesize that the creation of an iliac AVF would result in significant alterations to the limb muscle metabolome. Recently, our group developed a new murine model to address the pathophysiology of access-related hand dysfunction (ARHD) in mice, where AVF creation is performed in the iliac artery/vein. Because of the anatomical location of the AVF creation, this model produces clinically relevant changes in the mouse hindlimb including hemodynamic alterations, muscle weakness, and mitochondrial function impairment. |
Institute | University of Florida |
Department | Applied Physiology and Kinesiology |
Laboratory | Rm 42 and Rm 43 |
Last Name | Ryan |
First Name | Terence |
Address | 1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA |
ryant@ufl.edu | |
Phone | 352-294-1700 |
Submit Date | 2022-02-17 |
Num Groups | 4 |
Total Subjects | 34 |
Num Males | All |
Study Comments | Metabolomic study via NMR |
Publications | Frontiers |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2022-08-01 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002287 |
Treatment Summary: | Briefly, mice were acclimated to a standard casein-based chow (Control Diet) for 7 days. Thereafter, the animals were randomly assign to either remain on Control Diet (non-CKD groups) or receive adenine-supplemented diet to induce CKD. Following three weeks of the casein/adenine diet, animals were randomly assigned to receive either an arteriovenous fistula surgery (AVF) or sham surgery (SHAM) |
Animal Vet Treatments: | none |
Animal Anesthesia: | isoflurane |
Animal Fasting: | non-fasted |
Animal Endp Euthanasia: | Euthanasia was carried out by thoracotomy followed by cervical dislocation. |
Animal Endp Tissue Coll List: | Skeletal muscle (quadriceps) |