Summary of Study ST002189
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001395. The data can be accessed directly via it's Project DOI: 10.21228/M8DB02 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002189 |
| Study Title | Metabolomic profiling reveals muscle metabolic changes following iliac arteriovenous fistula creation in mice (Aqueous) |
| Study Type | Study of the skeletal muscle metabolome in mice with iliac arteriovenous fistula via 1H NMR |
| Study Summary | In the present study, we hypothesize that the creation of an iliac AVF would result in significant alterations to the limb muscle metabolome. Recently, our group developed a new murine model to address the pathophysiology of access-related hand dysfunction (ARHD) in mice, where AVF creation is performed in the iliac artery/vein. Because of the anatomical location of the AVF creation, this model produces clinically relevant changes in the mouse hindlimb including hemodynamic alterations, muscle weakness, and mitochondrial function impairment. |
| Institute | University of Florida |
| Department | Applied Physiology and Kinesiology |
| Laboratory | Rm 42 and Rm 43 |
| Last Name | Ryan |
| First Name | Terence |
| Address | 1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA |
| ryant@ufl.edu | |
| Phone | 352-294-1700 |
| Submit Date | 2022-02-17 |
| Num Groups | 4 |
| Total Subjects | 34 |
| Num Males | All |
| Study Comments | Metabolomic study via NMR |
| Publications | Frontiers |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2022-08-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002281 |
| Sampleprep Summary: | Weights of frozen quadriceps specimens were weighed using a microbalance (Mettler-Toledo, Columbus, OH, USA). Next, a slightly modified FOLCH extraction was performed to extract aqueous and lipid phase metabolites. The aqueous phase was lyophilized overnight (Labconco Corporation, Kansas, MO, USA) and the lipid phase was dried by passing inert nitrogen gas. The resulting aqueous and lipid phase dry powders were stored at -80oC until analysis using nuclear magnetic resonance (NMR). The dry powder of aqueous phase samples was dissolved in 50 µL of phosphate buffer system (50 mM, pH 7.2) consisting of 0.5 mM D6-DSS, 2 mM EDTA and 0.2% NaN2. Lipid phase dry powders were dissolved in 70 µL of CDCl3 supplemented with 10 mM of pyrazine (as internal NMR standard). All samples were loaded into 1.5 mm optical density (O.D.) NMR tubes. |
| Sampleprep Protocol Filename: | AVF_NMR_Aqueous_Phase_Procedures.docx |
| Processing Method: | Lyophilization and Homogenization |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Modified FOLCH extraction |
| Extract Storage: | -80℃ |
| Sample Resuspension: | In 50 microliter of 50 mM phosphate buffer (pH 7.2) with 2 mM EDTA, 0.5 mM DSS and 0.2% sodium azide for aqueous phase samples. |
| Sample Spiking: | 0.5 mM of DSS for aqueous phase samples |
