Summary of Study ST002189
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001395. The data can be accessed directly via it's Project DOI: 10.21228/M8DB02 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST002189 |
| Study Title | Metabolomic profiling reveals muscle metabolic changes following iliac arteriovenous fistula creation in mice (Aqueous) |
| Study Type | Study of the skeletal muscle metabolome in mice with iliac arteriovenous fistula via 1H NMR |
| Study Summary | In the present study, we hypothesize that the creation of an iliac AVF would result in significant alterations to the limb muscle metabolome. Recently, our group developed a new murine model to address the pathophysiology of access-related hand dysfunction (ARHD) in mice, where AVF creation is performed in the iliac artery/vein. Because of the anatomical location of the AVF creation, this model produces clinically relevant changes in the mouse hindlimb including hemodynamic alterations, muscle weakness, and mitochondrial function impairment. |
| Institute | University of Florida |
| Department | Applied Physiology and Kinesiology |
| Laboratory | Rm 42 and Rm 43 |
| Last Name | Ryan |
| First Name | Terence |
| Address | 1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA |
| ryant@ufl.edu | |
| Phone | 352-294-1700 |
| Submit Date | 2022-02-17 |
| Num Groups | 4 |
| Total Subjects | 34 |
| Num Males | All |
| Study Comments | Metabolomic study via NMR |
| Publications | Frontiers |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2022-08-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000241 |
| Analysis ID: | AN003583 |
| Instrument Name: | Bruker Avance III Cryo800 MHz 54 mm |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5 mM DSS |
| Spectrometer Frequency: | 799.9 |
| NMR Probe: | CP TXI CryoProbe |
| NMR Solvent: | Phosphate buffer (pH 7.2) + 2 mM EDTA + 0.5 mM DSS + 0.2% of sodium azide in deuterated environment |
| NMR Tube Size: | 1.5 mm O.D. |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | presat |
| Pulse Width: | 90-degree |
| Receiver Gain: | 645 |
| Offset Frequency: | 2827.31 Hz |
| Chemical Shift Ref Cpd: | DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid) |
| Temperature: | 25 o C |
| Number Of Scans: | 128 scans |
| Dummy Scans: | 8 |
| Acquisition Time: | 4 s |
| Relaxation Delay: | 1 s |
| Spectral Width: | 9523.8 |
| Num Data Points Acquired: | 38094 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.22 Hz |
| Zero Filling: | 65,536 points |
| Apodization: | Exponential |
| Baseline Correction Method: | Spline |
| Chemical Shift Ref Std: | 0 ppm for DSS |
| Binned Increment: | None |
| Binned Data Excluded Range: | None |
