Summary of Study ST000442
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000341. The data can be accessed directly via it's Project DOI: 10.21228/M8989V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000442 |
Study Title | Metabolomics Analysis of Triple Negative Breast Cancer (BCa) Cell Lines |
Study Type | Metabolomics comparison of different breast cancer cell lines |
Study Summary | We used untargeted metabolomic profiling to distinguish this form of BCa from estrogen receptor positive (ER+) subtypes (+/- HER2/neu) and determine that may explain why a commonly used chemotherapeutic, paclitaxel, is generally ineffective at eliciting long-term cytotoxic and/or cytostatic responses in cell line models of TNBC. This metabolomics study used broad spectrum 1H NMR to compare Luminal A (BT474, MCF-7) and triple-negative (MDA-MB-231, MDA-MB-468) BCa cell lines, to determine differences in the two subtypes as well as distinguish therapeutic treatment responses for identifying new targets for drug discovery. |
Institute | University of North Carolina |
Department | Discovery Sciences |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2016-08-02 |
Num Groups | 4 |
Total Subjects | 24 |
Study Comments | 4 breast cancer cell lines, treated with paclitaxel compared to controls (3 replicates/line/condition) |
Publications | J. Proteome Res., Article ASAP, DOI: 10.1021/acs.jproteome.6b00430 |
Raw Data Available | Yes |
Raw Data File Type(s) | 1r |
Analysis Type Detail | NMR |
Release Date | 2016-12-22 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR000477 |
Treatment Summary: | Briefly, mycoplasma tested and certified BCa cell lines will be purchased from American Type Culture Collection (ATCC) and maintained under the manufacturer’s recommended conditions. Cells were grown for 48 h in the presence or absence of chemotherapeutic agent paclitaxel (10 nM). |