Summary of Study ST000442
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000341. The data can be accessed directly via it's Project DOI: 10.21228/M8989V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000442 |
| Study Title | Metabolomics Analysis of Triple Negative Breast Cancer (BCa) Cell Lines |
| Study Type | Metabolomics comparison of different breast cancer cell lines |
| Study Summary | We used untargeted metabolomic profiling to distinguish this form of BCa from estrogen receptor positive (ER+) subtypes (+/- HER2/neu) and determine that may explain why a commonly used chemotherapeutic, paclitaxel, is generally ineffective at eliciting long-term cytotoxic and/or cytostatic responses in cell line models of TNBC. This metabolomics study used broad spectrum 1H NMR to compare Luminal A (BT474, MCF-7) and triple-negative (MDA-MB-231, MDA-MB-468) BCa cell lines, to determine differences in the two subtypes as well as distinguish therapeutic treatment responses for identifying new targets for drug discovery. |
| Institute | University of North Carolina |
| Department | Discovery Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2016-08-02 |
| Num Groups | 4 |
| Total Subjects | 24 |
| Study Comments | 4 breast cancer cell lines, treated with paclitaxel compared to controls (3 replicates/line/condition) |
| Publications | J. Proteome Res., Article ASAP, DOI: 10.1021/acs.jproteome.6b00430 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | 1r |
| Analysis Type Detail | NMR |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
| NMR ID: | NM000077 |
| Analysis ID: | AN000693 |
| Instrument Name: | Bruker Avance III 600 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5 mM DSS |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 5 mm ATMA Cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5 mm |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesyprid |
| Water Suppression: | yes |
| Pulse Width: | 9.28 us |
| Power Level: | 39.811 W |
| Receiver Gain: | 50.8 |
| Offset Frequency: | 2817.50 Hz |
| Presaturation Power Level: | 0.000025119 W |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 298.1 K |
| Number Of Scans: | 256 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.482 s |
| Relaxation Delay: | 2 s |
| Spectral Width: | 11.0011 ppm, 6602.113 Hz |
| Num Data Points Acquired: | 32768 |
| Real Data Points: | 16384 |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS |
| Binned Increment: | 0.04 |
| Binned Data Excluded Range: | water (4.66 - 4.86 ppm); imidazole (7.15-7.26 ppm) |
