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MB Sample ID: SA005403

Local Sample ID:T18-3
Subject ID:SU000114
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:9 months
Animal Housing:Singly housed
Animal Feed:High fat diet (HFD, 4.73 kcal/g, 20% protein, 35% carbohydrate, and 45% fat per kcal; D12451, Research Diets, New Brunswick, NJ)
Species Group:Mammal

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Combined analysis:

Analysis ID AN000151
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity UPLC
Column Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS instrument type Triple quadrupole
MS instrument name ABI Sciex API 6500 QTrap
Units % abundance


Chromatography ID:CH000107
Chromatography Summary:Targeted UPLC-MS/MS method
Methods Filename:Newman_Lab_Bile_Acids_Extraction_&_Analysis_Protocol_La_Merrill_WCMC_P&F.pdf
Instrument Name:Waters Acquity UPLC
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Column Temperature:60 C
Flow Gradient:Yes
Flow Rate:0.4mL/min
Internal Standard:See protocol/methods file
Sample Injection:5 L
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:16 min
Weak Wash Solvent Name:20% methanol, 10% isopropanol
Weak Wash Volume:600 L
Strong Wash Solvent Name:50:50 Acetonitrile:Methanol
Strong Wash Volume:600 L
Sample Loop Size:17 L
Chromatography Type:Reversed phase