Return to study ST001670 main page
MB Sample ID: SA153386
| Local Sample ID: | BB 02030 metabo FA 005 |
| Subject ID: | SU001747 |
| Subject Type: | Fish |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002725 | AN002726 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | Reversed phase |
| Chromatography system | Vanquish UHPLC (Thermo Fisher Scientific). | Thermo Vanquish UHPLC |
| Column | Waters Xbridge amide HILIC (100 x 2.1mm,3.5um) | Luna C18 LC (150 x 4.6 mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | Ion trap | Ion trap |
| MS instrument name | Thermo Orbitrap Exploris 480 | Thermo Orbitrap Exploris 480 |
| Ion Mode | UNSPECIFIED | UNSPECIFIED |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002011 |
| Chromatography Summary: | For this polar metabolite analysis, a hydrophilic interaction chromatography method (HILIC) with an Xbridge amide column (100 x 2.1 mm i.d., 3.5 µm; Waters) was used for compound separation at 25 °C. Mobile phase A: water with 5 mM ammonium acetate (pH 6.8), and mobile phase B: 100 % acetonitrile. Linear gradient was: 0 min, 85% B; 1.5 min, 85% B; 5.5 min, 35% B; 6.9 min, 35% B; 10.5 min, 35% B; 10.6 min, 10% B; 12.5 min, 10% B; 13.5 min, 85% B; 17.9 min, 85% B; 18 min, 85% B; 20 min, 85% B. The flow rate was: 0-5.5 min, 0.11 ml/min; 6.9-10.5 min, 0.13 ml/min; 10.6-17.9 min, 0.25 ml/min; 18-20 min, 0.11 ml/min. |
| Instrument Name: | Vanquish UHPLC (Thermo Fisher Scientific). |
| Column Name: | Waters Xbridge amide HILIC (100 x 2.1mm,3.5um) |
| Column Temperature: | 25 |
| Flow Gradient: | 0 min, 85% B; 1.5 min, 85% B; 5.5 min, 35% B; 6.9 min, 35% B; 10.5 min, 35% B; 10.6 min, 10% B; 12.5 min, 10% B; 13.5 min, 85% B; 17.9 min, 85% B; 18 min, 85% B; 20 min, 85% B. |
| Flow Rate: | 0-5.5 min, 0.11 ml/min; 6.9-10.5 min, 0.13 ml/min; 10.6-17.9 min, 0.25 ml/min; 18-20 min, 0.11 ml/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 6.8 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002012 |
| Chromatography Summary: | The analysis of folic acid and its derivatives was performed using Luna column (100 x 3 mm i.d., 3 µm, C18(2), 100 Å, Phenomenex) at 25 °C. Mobile phase A: water with 0.1% formic acid, and mobile phase B: 100 % methanol. Linear gradient was: 0 min, 2% B; 1.5 min, 2% B; 3.0 min, 15% B; 5.5 min, 95% B; 14.5 min, 95% B; 15.0 min, 2% B; 22.0 min, 2% B. The flow rate was 0.150 ml/min. |
| Chromatography Comments: | Luna® 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea |
| Instrument Name: | Thermo Vanquish UHPLC |
| Column Name: | Luna C18 LC (150 x 4.6 mm,3um) |
| Column Temperature: | 25 |
| Flow Gradient: | 0 min, 2% B; 1.5 min, 2% B; 3.0 min, 15% B; 5.5 min, 95% B; 14.5 min, 95% B; 15.0 min, 2% B; 22.0 min, 2% B. |
| Flow Rate: | 0.150 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
