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MB Sample ID: SA153386

Local Sample ID:BB 02030 metabo FA 005
Subject ID:SU001747
Subject Type:Fish
Subject Species:Danio rerio
Taxonomy ID:7955

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Combined analysis:

Analysis ID AN002725 AN002726
Analysis type MS MS
Chromatography type HILIC Reversed phase
Chromatography system Vanquish UHPLC (Thermo Fisher Scientific). Thermo Vanquish UHPLC
Column Waters Xbridge amide HILIC (100 x 2.1mm,3.5um) Luna C18 LC (150 x 4.6 mm,3um)
MS Type ESI ESI
MS instrument type Ion trap Ion trap
MS instrument name Thermo Orbitrap Exploris 480 Thermo Orbitrap Exploris 480
Ion Mode UNSPECIFIED UNSPECIFIED
Units peak area peak area

MS:

MS ID:MS002522
Analysis ID:AN002725
Instrument Name:Thermo Orbitrap Exploris 480
Instrument Type:Ion trap
MS Type:ESI
MS Comments:Xcalibur 4.4 from Thermo Scientific for data acquisition and we use Sieve 2.2 for chromatographic alignment and peak integration.To analyze polar metabolites, the mass spectrometer was equipped with a HESI probe and operated in the positive/negative switching mode. The relevant parameters were as listed: vaporizer temperature, 350 °C; ion transfer tube temperature, 300 °C; sheath gas, 35; auxiliary gas, 7; sweep gas, 1; spray voltage, 3.5 kV for positive mode and 2.5 kV for negative mode; RF-lens (%), 30. The resolution was set at 60,000 (at m/z 200). Automatic maximum injection time (max IT) and automated gain control (AGC) were used.
Ion Mode:UNSPECIFIED
  
MS ID:MS002523
Analysis ID:AN002726
Instrument Name:Thermo Orbitrap Exploris 480
Instrument Type:Ion trap
MS Type:ESI
MS Comments:Xcalibur 4.4 from Thermo Scientific for data acquisition and we use Sieve 2.2 for chromatographic alignment and peak integration.To measure folate derivatives, Orbitrap Exploris 480 was operated in SIM mode (selected ion monitoring) targeting the following ions: folic acid (442.1470), 5-methyltetrahydrofolate (460.1939), and folinic acid (474.1732).
Ion Mode:UNSPECIFIED
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