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MB Sample ID: SA176934
| Local Sample ID: | OB+BMJ_04(raw) |
| Subject ID: | SU001989 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wildtype |
| Age Or Age Range: | 4 weeks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory |
| Animal Housing: | UC Denver |
| Animal Feed: | High fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492 |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003108 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Agilent 6210 TOF |
| Ion Mode | POSITIVE |
| Units | Abundance (mass spectral counts) |
Chromatography:
| Chromatography ID: | CH002295 |
| Chromatography Summary: | The samples from the lipid fraction were analyzed an Agilent 1290 series pump with an Agilent Zorbax Rapid Resolution HD (RRHD) SB-C18, 1.8 micron (2.1 x 100 mm) analytical column and an Agilent Zorbax SB-C18, 1.8 micron (2.1 x 5 mm) guard column. The autosampler tray temperature was set at 4°C, column temperature was set at 60°C, and the sample injection volume was 4µL. The flow rate was 0.7 mL/min with the following mobile phases: mobile phase A was water with 0.1% formic acid, and mobile phase B was 60:36:4 isopropyl alcohol:acetonitrile:water with 0.1% formic acid. Gradient elution was as follows: 0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes. |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| Column Temperature: | 60 |
| Flow Gradient: | 0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes. |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 60% isopropanol/36% acetonitrile/4% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
