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MB Sample ID: SA176934
| Local Sample ID: | OB+BMJ_04(raw) |
| Subject ID: | SU001989 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wildtype |
| Age Or Age Range: | 4 weeks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory |
| Animal Housing: | UC Denver |
| Animal Feed: | High fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492 |
| Animal Water: | ad libitum |
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Sample Preparation:
| Sampleprep ID: | SP001995 |
| Sampleprep Summary: | Frozen plasma was stored at -80°C until sample preparation for untargeted liquid-chromatography mass spectrometry (LC-MS)-based metabolomics. Briefly, 100µL of sample underwent protein precipitation using methanol, followed by liquid-liquid extraction using methyl-tert butyl ether as previously described[1, 2] to obtain an aqueous fraction and a lipid fraction. The lipid fraction was dried down and resuspended in 100% methanol for LC-MS analysis. |
| Processing Storage Conditions: | Described in summary |
