Summary of Study ST000667

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000470. The data can be accessed directly via it's Project DOI: 10.21228/M89G75 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000667
Study TitleDiabetic Microvascular Plasma/Tissue Lipidomics- Comparison
Study TypeMS analysis
Study SummaryDetermining lipid composition of diabetic microvascular complication-prone tissues and comparing tissues levels to plasma levels. Samples are in addition to plasma and kidney tissue samples ran (shotgun lipidomics) in June-July 2014.
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups6
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR000470
Project DOI:doi: 10.21228/M89G75
Project Title:Diabetic Microvascular Plasma/Tissue Lipidomics- Comparison
Project Type:MS analysis
Project Summary:Determine lipids in plasma and microvascular complication prone tissues in diabetic mouse models
Institute:University of Michigan
Department:UMich Internal Medicine
Laboratory:Pennathur Lab
Last Name:Pennathur
First Name:Subramaniam
Address:University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Phone:734 615 5757


Subject ID:SU000690
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal


Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id CONTROL_DIABETIC Sample type
SA038424S00018519db/db diabetic Kidney
SA038425S00018520db/db diabetic Kidney
SA038426S00018521db/db diabetic Kidney
SA038427S00018523db/db diabetic Plasma
SA038428S00018525db/db diabetic Plasma
SA038429S00018522db/db diabetic Plasma
SA038430S00018524db/db diabetic Plasma
SA038431S00018516db/db diabetic Retina
SA038432S00018517db/db diabetic Retina
SA038433S00018515db/db diabetic Retina
SA038434S00018514db/db diabetic Retina
SA038435S00018508db/db diabetic Sciatic Nerve
SA038436S00018504db/db diabetic Sciatic Nerve
SA038437S00018505db/db diabetic Sciatic Nerve
SA038438S00018506db/db diabetic Sciatic Nerve
SA038439S00018507db/db diabetic Sciatic Nerve
SA038440S00018518db/m control Kidney
SA038441S00018526db/m control Plasma
SA038442S00018513db/m control Retina
SA038443S00018510db/m control Retina
SA038444S00018511db/m control Retina
SA038445S00018509db/m control Retina
SA038446S00018512db/m control Retina
SA038447S00018500db/m control Sciatic Nerve
SA038448S00018501db/m control Sciatic Nerve
SA038449S00018503db/m control Sciatic Nerve
SA038450S00018499db/m control Sciatic Nerve
SA038451S00018502db/m control Sciatic Nerve
Showing results 1 to 28 of 28


Collection ID:CO000684
Collection Summary:-
Sample Type:Blood


Treatment ID:TR000704
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000697
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001020 AN001021
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Units peak area normalized peak area normalized


Chromatography ID:CH000730
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase


MS ID:MS000915
Analysis ID:AN001020
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
MS ID:MS000916
Analysis ID:AN001021
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf