Summary of Study ST000095
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000087. The data can be accessed directly via it's Project DOI: 10.21228/M8F30Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000095 |
Study Title | Dysfunctional lipid metabolism underlies the effect of perinatal DDT exposure on the development of metabolic syndrome |
Study Type | Chemical dosage and feeding study |
Study Summary | Targeted metabolomic analysis of bile acids was performed on 15 mouse liver samples collected from mice euthanized at 9 months following consumption of a high fat diet w/o perinatal DDT exposure. Funded by the National Institute of Health (R00 ES019919, R03 DK082724, U24 DK092993, U24 DK097154, T32 ES007059, and P60 DK020541), the American Diabetes Association, and USDA-ARS intramural Project 5306-51530-019-00D. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization. |
Institute | University of California, Davis |
Department | U.S.D.A. Western Human Nutrition Research Center |
Laboratory | Newman |
Last Name | Newman |
First Name | John |
Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
john.newman@ars.usda.gov | |
Phone | +1-530-752-1009 |
Submit Date | 2014-07-10 |
Num Groups | 2 |
Total Subjects | 15 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Uploaded File Size | 23 M |
Analysis Type Detail | LC-MS |
Release Date | 2015-02-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000151 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity UPLC |
Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex API 6500 QTrap |
Ion Mode | NEGATIVE |
Units | % abundance |
Chromatography:
Chromatography ID: | CH000107 |
Chromatography Summary: | Targeted UPLC-MS/MS method |
Methods Filename: | Newman_Lab_Bile_Acids_Extraction_&_Analysis_Protocol_La_Merrill_WCMC_P&F.pdf |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 60 C |
Flow Gradient: | Yes |
Flow Rate: | 0.4mL/min |
Internal Standard: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Analytical Time: | 16 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |
Chromatography Type: | Reversed phase |