Summary of Study ST000095
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000087. The data can be accessed directly via it's Project DOI: 10.21228/M8F30Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000095 |
| Study Title | Dysfunctional lipid metabolism underlies the effect of perinatal DDT exposure on the development of metabolic syndrome |
| Study Type | Chemical dosage and feeding study |
| Study Summary | Targeted metabolomic analysis of bile acids was performed on 15 mouse liver samples collected from mice euthanized at 9 months following consumption of a high fat diet w/o perinatal DDT exposure. Funded by the National Institute of Health (R00 ES019919, R03 DK082724, U24 DK092993, U24 DK097154, T32 ES007059, and P60 DK020541), the American Diabetes Association, and USDA-ARS intramural Project 5306-51530-019-00D. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization. |
| Institute | University of California, Davis |
| Department | U.S.D.A. Western Human Nutrition Research Center |
| Laboratory | Newman |
| Last Name | Newman |
| First Name | John |
| Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
| john.newman@ars.usda.gov | |
| Phone | +1-530-752-1009 |
| Submit Date | 2014-07-10 |
| Num Groups | 2 |
| Total Subjects | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Uploaded File Size | 23 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-02-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000110 |
| Sampleprep Summary: | A 15 mg liver sample (n=1 female/litter in 7 VEH and 8 DDT litters) was pulverized on dry ice, enriched with deuterated bile acid surrogates, butylated hydroxytoluene and ethylinediaminetetraacetic acid, and extracted twice with 500 uL methanol. The combined extract was dried, reconstituted in 100uL 50:50 methanol:acetonitrile with internal standards 1-phenyl-3-hexanoic acid urea and 1-cyclohexyl-3-dodecanoic acid urea (Sigma-Aldrich, St. Louis, MO) and filtered at 0.1 µm. |
| Sampleprep Protocol Filename: | Newman_Lab_Bile_Acid_Protocols_La_Merrill_WCMC_P&F.pdf |
| Processing Method: | Pulverization on dry ice |
| Extraction Method: | Methanol |
| Extract Concentration Dilution: | Dried with Rotoevaporator |
| Extract Storage: | Placed in 10 °C autosampler and immediately analyzed |
| Sample Spiking: | Spiked with deuterated bile acid surrogates. |
| Organ: | Liver |
