Summary of Study ST000254
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000206. The data can be accessed directly via it's Project DOI: 10.21228/M8RW2F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
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Study ID | ST000254 |
Study Title | The role of microbial metabolites in experimental liver disease |
Study Type | Targeted Metabolomic Analysis of plasma samples |
Study Summary | Aim 1: Our experimental approach is to understand the effect of drinking water supplemented bacterial metabolite, Indole-3-propionic Acid (IPA), in liver disease in an acute alcohol model. Aim 2: Determine the levels of IPA in plasma of conventional mice in a chronic alcohol model. Aim 3: Determine the levels of IPA in plasma of conventional WT (C57BL/6) and mutant SL (sublytic, which is a mouse with a point mutation in ATP4a) mice. |
Institute | University of North Carolina |
Department | Discovery Science Technology |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2015-09-29 |
Num Groups | 2 |
Total Subjects | 31 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Uploaded File Size | 8.6 M |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000402 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex API 4000 QTrap |
Ion Mode | NEGATIVE |
Units |
Chromatography:
Chromatography ID: | CH000284 |
Chromatography Summary: | Reversed-Phase Gradient Seperation |
Methods ID: | RTI-RCMRC-IPA-Analysis |
Methods Filename: | RTI-RCMRC-IPA-Analysis |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
Column Pressure: | 6000-10000 |
Column Temperature: | 50 C |
Flow Gradient: | Time(min) Flow Rate %A %B Curve ;Initial 0.600 99.0 1.0 6;0.75 0.600 99.0 1.0 6 ;2.75 0.600 1.0 99.0 6 ;3.00 0.600 1.0 99.0 6 ;3.10 0.600 99.0 1.0 6 ;3.50 0.600 99.0 1.0 6; |
Flow Rate: | 0.6 ml/min |
Injection Temperature: | 8 C |
Internal Standard: | 2-methyl-4-chlorophenyl-acetic acid (MCPA) |
Solvent A: | 95% water/5% acetonitrile; 0.15% acetic acid |
Solvent B: | 95% acetonitrile/5% water; 0.15% acetic acid |
Analytical Time: | 3.5 mins |
Weak Wash Solvent Name: | 95:5 Water:ACN with 0.15% Acetic Acid |
Weak Wash Volume: | 600 uL |
Strong Wash Solvent Name: | 95:5 ACN:Water with 0.15% Acetic Acid |
Strong Wash Volume: | 600 uL |
Target Sample Temperature: | 8 C |
Sample Loop Size: | 10 uL |
Sample Syringe Size: | 100 uL |
Randomization Order: | Yes |
Chromatography Type: | Reversed phase |