Summary of Study ST000254
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000206. The data can be accessed directly via it's Project DOI: 10.21228/M8RW2F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
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Study ID | ST000254 |
Study Title | The role of microbial metabolites in experimental liver disease |
Study Type | Targeted Metabolomic Analysis of plasma samples |
Study Summary | Aim 1: Our experimental approach is to understand the effect of drinking water supplemented bacterial metabolite, Indole-3-propionic Acid (IPA), in liver disease in an acute alcohol model. Aim 2: Determine the levels of IPA in plasma of conventional mice in a chronic alcohol model. Aim 3: Determine the levels of IPA in plasma of conventional WT (C57BL/6) and mutant SL (sublytic, which is a mouse with a point mutation in ATP4a) mice. |
Institute | University of North Carolina |
Department | Discovery Science Technology |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2015-09-29 |
Num Groups | 2 |
Total Subjects | 31 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Uploaded File Size | 8.6 M |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000280 |
Sampleprep Summary: | Aliquots of plasma samples (20 µL) were transferred into pre-labeled 2.0 mL low-bind Eppendorf tubes for experimental samples. For all samples 100 µL Protein Precipitation Buffer with internal standard [2.5 ng/ml of 2-methyl-4-chlorophenyl-acetic acid (MCPA) in methanol] was added to each tube and were vortexed for 2 min at 5000 rpm. The samples were centrifuged at 16,000 rcf for 4 min. A 90 µL of the supernatant wastransferred into pre-labeled auto-sampler vials and diluted by adding 90 µL of water with 10% Formic Acid. Samples were then mixed on a multiple tube vortexer for 5 min at 5000 rpm. 10 µL of samples were injected onto API-4000 Q-trap. |
Processing Storage Conditions: | 4°C |
Extract Storage: | -80C |
Sample Resuspension: | Methanol, H2O, formic acid |
Sample Spiking: | 2-methyl-4-chlorophenyl-acetic acid (MCPA) |