Summary of Study ST001824
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001153. The data can be accessed directly via it's Project DOI: 10.21228/M8P10F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001824 |
| Study Title | Metabolomic and lipidomic profiles of CKD in obese patients in serum and urine (part 1 of 3) |
| Study Type | Human nephropathy in CKD obese patients |
| Study Summary | Obesity is a global pandemic with an increase prevalence over the years. This condition elevates the risk of developing cardiovascular diseases, hypertension and renal pathologies, like chronic kidney disease (CKD). In the present study, the metabolomic and the lipidomic profiles of CKD obese patients were analyzed comparing with obese subjects without CKD. Subsequently, CKD obese patients underwent bariatric surgery and the effect of surgery in the CKD progression of these subjects was evaluated. Serum and urine were measured by LC-MS and GC-HRAM equipment. |
| Institute | University Rey Juan Carlos |
| Department | Basics Science of Health |
| Last Name | Lanzon |
| First Name | Borja |
| Address | Avenida de Atenas S/N |
| borja.lanzon@urjc.es | |
| Phone | 663692554 |
| Submit Date | 2021-04-02 |
| Num Groups | 3 |
| Total Subjects | 36 |
| Study Comments | Serum LC-MS data: part 1 of 3. Samples were analyzed per duplicated. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-06-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002960 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent Infinity 1290 |
| Column | Waters ACQUITY UPLC BEH C18 |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6545 Q-TOF |
| Ion Mode | POSITIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH002193 |
| Chromatography Summary: | An ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight (UHPLC-ESI-Q-TOF-MS) were used to analyze the samples on positive ionization mode. Samples were analyzed by duplicate. UHPLC system was an Agilent Infinity 1290 provided by Agilent Technologies (Santa Clara, CA, USA) equipped with a multisampler (kept at -10 °C). Needle wash solutions were performed with 10% DCM in MeOH and ACN:MeOH:IPA:H2O (1:1:1:1, v/v/v/v), and 0.1% of HCOOH after each injection for 7.5 s. System were equipped with a column thermostat (maintained at 50 °C) and a quaternary solvent manager. An ACQUITY UPLC BEH C18 column was used for separations (2.1 mm x 100 mm, particle size 1.7 μm) purchased at Waters (Milford, CT, USA). Injection volume was 1 μl and flow rate was established at 0.4 ml/min. Mobile phases were composed of (A) H2O + NH4AC 10 mM + 0.1 % HCOOH and (B) ACN: IPA (1:1, v/v) + NH4AC 10 mM + 0.1 % HCOOH. Gradient was from 0 to 2 min 35-80 % B, 2 min to 7 min 80-100 % B and 7 to 14 min 100 % B. A re-equilibration of 7 min was performed after each run to bring system to initial conditions (35 % B). |
| Instrument Name: | Agilent Infinity 1290 |
| Column Name: | Waters ACQUITY UPLC BEH C18 |
| Column Temperature: | 50 |
| Flow Gradient: | 0 to 2 min 35-80 % B, 2 min to 7 min 80-100 % B and 7 to 14 min 100 % B |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium acetate |
| Solvent B: | 50% acetonitrile/50% isopropanol; 0.1% formic acid; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
