Summary of Study ST001824
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001153. The data can be accessed directly via it's Project DOI: 10.21228/M8P10F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001824 |
| Study Title | Metabolomic and lipidomic profiles of CKD in obese patients in serum and urine (part 1 of 3) |
| Study Type | Human nephropathy in CKD obese patients |
| Study Summary | Obesity is a global pandemic with an increase prevalence over the years. This condition elevates the risk of developing cardiovascular diseases, hypertension and renal pathologies, like chronic kidney disease (CKD). In the present study, the metabolomic and the lipidomic profiles of CKD obese patients were analyzed comparing with obese subjects without CKD. Subsequently, CKD obese patients underwent bariatric surgery and the effect of surgery in the CKD progression of these subjects was evaluated. Serum and urine were measured by LC-MS and GC-HRAM equipment. |
| Institute | University Rey Juan Carlos |
| Department | Basics Science of Health |
| Last Name | Lanzon |
| First Name | Borja |
| Address | Avenida de Atenas S/N |
| borja.lanzon@urjc.es | |
| Phone | 663692554 |
| Submit Date | 2021-04-02 |
| Num Groups | 3 |
| Total Subjects | 36 |
| Study Comments | Serum LC-MS data: part 1 of 3. Samples were analyzed per duplicated. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-06-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002960 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent Infinity 1290 |
| Column | Waters ACQUITY UPLC BEH C18 |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6545 Q-TOF |
| Ion Mode | POSITIVE |
| Units | Area |
MS:
| MS ID: | MS002750 |
| Analysis ID: | AN002960 |
| Instrument Name: | Agilent 6545 Q-TOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Mass Spectrometer was an Agilent 6545 quadrupole time-of-flight (Q-TOF) mounted with a dual jet stream electrospray (dual ESI) ion source interface. Nitrogen was obtained from a nitrogen generator (PEAK Scientific, Renfrewshire, Scotland, UK) as a sheath gas at a flow rate of 11 l/min at 379 °C. As a Collision gas was used Pure Nitrogen (6.0) from Praxair (Fredericia, Denmark). Capillary voltage was maintained at 3600 V and nozzle voltage was kept at 1500 V. Reference mass solution was prepared in consonance with Agilent guidelines, including ions at m/z 121.0509 and 922.0098. The second nebulizer was used to introduce the solution in the dual ESI ion source through the isocratic pump at a constant flow rate of 4 ml/min (split to 1:100 before nebulization). Acquisition mass range was 100 to 3000 m/z. Instrument used the extended dynamic range with an estimated resolution of 30.000 FWHM measured at 1521.9715 m/z (included in tune mixture) when instrument calibration was performed. |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3600 V |
