Summary of Study ST001904
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001199. The data can be accessed directly via it's Project DOI: 10.21228/M8QQ5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001904 |
| Study Title | Lipidomics analysis of outer membrane vesicles and elucidation of the ceramide phosphoinositol biosynthetic pathway in Bacteroides thetaiotaomicron |
| Study Type | Lipidic profile in wild-type and mutant strains |
| Study Summary | In this work, we characterized the lipid composition of membranes and OMV from Bacteroides thetaiotaomicron VPI-5482. LC-MS analysis indicate that OMV carry sphingolipids, glycerophospholipids and serine-dipeptide lipids. Sphingolipid species represent more than 50% of the total lipid content of OMV. The most abundant sphingolipids in OMV are ceramide phosphoethanolamine (CerPE) and ceramide phosphoinositol (CerPI). Bioinformatic analysis allowed the identification of the BT1522-1526 operon putatively involved in CerPI synthesis. Mutagenesis studies revealed BT1522-1526 are essential for synthesis of PI and CerPI, confirming the role of this operon in biosynthesis of CerPI. BT1522-1526 mutant strains lacking CerPI produced OMV that were indistinguishable from the wild-type strain, indicating that CerPI sphingolipid species are not involved in OMV biogenesis. Bacteroides sphingolipids are thought to modulate host-commensal interactions, and based on our data, we propose that OMV could act as long distance delivery vehicles for these molecules. |
| Institute | Washington University in St. Louis |
| Department | Molecular Microbiology |
| Laboratory | Feldman lab |
| Last Name | Sartorio |
| First Name | Mariana |
| Address | 660 S Euclid avenue, campus box 8230, 63110 |
| mgsartorio@wustl.edu | |
| Phone | 3147474477 |
| Submit Date | 2021-06-22 |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003101 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6550 |
| Column | Thermo Betasil C18 (100 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | UNSPECIFIED |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH002288 |
| Chromatography Summary: | LC/MS analyses were conducted on an Agilent 6550 A QTOF instrument with an Agilent 1290 HPLC with autosampler, operated by Agilent Masshunter software (Santa Clara, CA USA). Separation of the total lipid extracts was achieved by a Thermo (Waltham MA, USA) 100 × 2.1 mm BETASIL 5 μm C18 column at a flow rate of 300 μl/min at room temperature. The mobile phase contained 5 mM ammonium formate (pH 5.0) both in solvent A, acetonitrile:water (60:40, v/v), and solvent B, isopropanol:acetonitrile (90:10, v/v). A gradient elution in the following manner was applied: 68% A, 0–1.5 min; 68–55% A, 1.5–4 min; 55–48% A, 4–5 min; 48–42% A, 5–8 min; 42–34% A, 8–11 min; 34–30% A, 11–14 min; 30–25% A, 14–18 min; 25–3% A, 18–23 min; 3–0% A, 25–30 min; 0% A, 30–35 min; 68% A, 35–40 min. Both the positive-ion and negative-ion ESI MS scans were acquired in the mass range of 200-2000 Da at a rate of 2 scans/min. |
| Instrument Name: | Agilent 6550 |
| Column Name: | Thermo Betasil C18 (100 x 2.1mm,5um) |
| Column Temperature: | RT |
| Flow Gradient: | 68% A, 0-1.5 min; 68-55% A, 1.5-4 min; 55-48% A, 4-5 min; 48-42% A, 5-8 min; 42-34% A, 8-11 min; 34-30% A, 11-14 min; 30-25% A, 14-18 min; 25-3% A, 18-23 min; 3-0% A, 25-30 min; 0% A, 30-35 min; 68% A, 35-40 min |
| Flow Rate: | 300ul/min |
| Solvent A: | 60% acetonitrile/40% water; 5 mM ammonium formate, pH 5.0 |
| Solvent B: | 90% isopropanol/10% acetonitrile |
| Chromatography Type: | Reversed phase |
