Summary of Study ST001904
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001199. The data can be accessed directly via it's Project DOI: 10.21228/M8QQ5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001904 |
| Study Title | Lipidomics analysis of outer membrane vesicles and elucidation of the ceramide phosphoinositol biosynthetic pathway in Bacteroides thetaiotaomicron |
| Study Type | Lipidic profile in wild-type and mutant strains |
| Study Summary | In this work, we characterized the lipid composition of membranes and OMV from Bacteroides thetaiotaomicron VPI-5482. LC-MS analysis indicate that OMV carry sphingolipids, glycerophospholipids and serine-dipeptide lipids. Sphingolipid species represent more than 50% of the total lipid content of OMV. The most abundant sphingolipids in OMV are ceramide phosphoethanolamine (CerPE) and ceramide phosphoinositol (CerPI). Bioinformatic analysis allowed the identification of the BT1522-1526 operon putatively involved in CerPI synthesis. Mutagenesis studies revealed BT1522-1526 are essential for synthesis of PI and CerPI, confirming the role of this operon in biosynthesis of CerPI. BT1522-1526 mutant strains lacking CerPI produced OMV that were indistinguishable from the wild-type strain, indicating that CerPI sphingolipid species are not involved in OMV biogenesis. Bacteroides sphingolipids are thought to modulate host-commensal interactions, and based on our data, we propose that OMV could act as long distance delivery vehicles for these molecules. |
| Institute | Washington University in St. Louis |
| Department | Molecular Microbiology |
| Laboratory | Feldman lab |
| Last Name | Sartorio |
| First Name | Mariana |
| Address | 660 S Euclid avenue, campus box 8230, 63110 |
| mgsartorio@wustl.edu | |
| Phone | 3147474477 |
| Submit Date | 2021-06-22 |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-30 |
| Release Version | 1 |
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Project:
| Project ID: | PR001199 |
| Project DOI: | doi: 10.21228/M8QQ5J |
| Project Title: | Lipidomic study |
| Project Type: | LC-MS analysis |
| Project Summary: | Lipidomic analysis of total membranes and outer membrane vesicles from the human gut comensal Bacteroides thetaiotaomicron. Strains used to perform the analysis: wild-tipe and mutants lacking the genes BT1522, BT1523, BT1524 and BT1526, involved in the synthesis of phosphoinosytol and Ceramide phosphinosytol |
| Institute: | Washington University in St. Louis |
| Department: | Molecular Microbiology |
| Laboratory: | Feldman lab |
| Last Name: | Sartorio |
| First Name: | Mariana |
| Address: | 660 S Euclid avenue, campus box 8230, 63110 |
| Email: | mgsartorio@wustl.edu |
| Phone: | 3147474477 |
| Funding Source: | NIH |
| Publications: | Lipidomics analysis of outer membrane vesicles and elucidation of the ceramide phosphoinositol biosynthetic pathway in Bacteroides thetaiotaomicron |
| Contributors: | Ezequiel Valguarnera, Mariana G. Sartorio, Fong-Fu Hsu and Mario F. Feldman |
