Summary of Study ST001911
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001205. The data can be accessed directly via it's Project DOI: 10.21228/M8Z700 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001911 |
| Study Title | A high-fat diet induced obesity murine model treated with bitter melon (Momordica charantia) |
| Study Type | A 40-day Intervention experiment |
| Study Summary | Metabolic effects of lyophilized bitter melon juice (BMJ) extract (oral gavage 200mg/kg/body weight-daily for 40 days) intake were evaluated in diet-induced obese C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat]. Changes in metabolite abundance levels in lipid-phase plasma [liquid chromatography mass spectrometry (LC-MS)-based metabolomics] after BMJ intervention were assessed. |
| Institute | University of Colorado Anschutz Medical Campus |
| Department | Pharmaceutical Sciences |
| Laboratory | Dr. Rajesh Agarwal's Laboratory |
| Last Name | Agarwal |
| First Name | Rajesh |
| Address | 12850 E. Montview Blvd, C238, Room V20-2118, Aurora, CO 80045, USA |
| Rajesh.Agarwal@cuanschutz.edu | |
| Phone | 303-724-4055 |
| Submit Date | 2021-05-22 |
| Num Groups | 2 |
| Total Subjects | 8 |
| Num Males | 8 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003108 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Agilent 6210 TOF |
| Ion Mode | POSITIVE |
| Units | Abundance (mass spectral counts) |
Chromatography:
| Chromatography ID: | CH002295 |
| Chromatography Summary: | The samples from the lipid fraction were analyzed an Agilent 1290 series pump with an Agilent Zorbax Rapid Resolution HD (RRHD) SB-C18, 1.8 micron (2.1 x 100 mm) analytical column and an Agilent Zorbax SB-C18, 1.8 micron (2.1 x 5 mm) guard column. The autosampler tray temperature was set at 4°C, column temperature was set at 60°C, and the sample injection volume was 4µL. The flow rate was 0.7 mL/min with the following mobile phases: mobile phase A was water with 0.1% formic acid, and mobile phase B was 60:36:4 isopropyl alcohol:acetonitrile:water with 0.1% formic acid. Gradient elution was as follows: 0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes. |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| Column Temperature: | 60 |
| Flow Gradient: | 0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes. |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 60% isopropanol/36% acetonitrile/4% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
