Summary of Study ST001911
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001205. The data can be accessed directly via it's Project DOI: 10.21228/M8Z700 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001911 |
| Study Title | A high-fat diet induced obesity murine model treated with bitter melon (Momordica charantia) |
| Study Type | A 40-day Intervention experiment |
| Study Summary | Metabolic effects of lyophilized bitter melon juice (BMJ) extract (oral gavage 200mg/kg/body weight-daily for 40 days) intake were evaluated in diet-induced obese C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat]. Changes in metabolite abundance levels in lipid-phase plasma [liquid chromatography mass spectrometry (LC-MS)-based metabolomics] after BMJ intervention were assessed. |
| Institute | University of Colorado Anschutz Medical Campus |
| Department | Pharmaceutical Sciences |
| Laboratory | Dr. Rajesh Agarwal's Laboratory |
| Last Name | Agarwal |
| First Name | Rajesh |
| Address | 12850 E. Montview Blvd, C238, Room V20-2118, Aurora, CO 80045, USA |
| Rajesh.Agarwal@cuanschutz.edu | |
| Phone | 303-724-4055 |
| Submit Date | 2021-05-22 |
| Num Groups | 2 |
| Total Subjects | 8 |
| Num Males | 8 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-30 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR002001 |
| Treatment Summary: | Sixteen-week-old diet-induced obese (DIO) C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492) from 4 weeks of age] were purchased from Jackson labs (Bar Harbor, ME). Once received at the animal house facility in UC Denver, the mice were randomized into two groups (n=4/ group): a) Control-DIO group maintained on the 60 kcal% HFD for additional 40 days, and b) BMJ+DIO group maintained on 60 kcal% HFD fat for additional 40 days plus orally gavage with lyophilized bitter melon juice extract [(5mg per mouse; total volume of 100 μl, 200mg/kg body weight/ day), reconstituted in sterile deionized water immediately before use)] throughout the study. |
| Treatment: | Intervention |
| Treatment Compound: | Bitter melon juice |
| Treatment Route: | Oral gavage |
| Treatment Dose: | 200mg/kg body weight/ day |
| Animal Fasting: | On day 40 of study initiation, mice in control and treament groups were subjected to fasting for six hours prior to sacrifice. |
| Animal Endp Euthanasia: | On day 40, mice were sacrificed after CO2 asphyxiation and exsanguination. |
