Summary of Study ST001958
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001245. The data can be accessed directly via it's Project DOI: 10.21228/M8S985 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001958 |
| Study Title | Data on changes in lipid profiles during differentiation and maturation of human subcutaneous white adipocytes analyzed using chromatographic and bioinformatics tools |
| Study Summary | Three cell lines of Caucasian-derived subcutaneous preadipocytes were divided into five stages (stage-1 to stage-5) from subcutaneous preadipocytes to mature subcutaneous adipocytes filled with many lipid droplets. Lipids were extracted from cells in each stage and processed using untargeted liquid chromatography and Q-Exactive Orbitrap tandem mass spectrometry. The lipids were identified using LipidSearch 4.2.13. |
| Institute | Hamamatsu University School of Medicine |
| Last Name | Kitamoto |
| First Name | Takuya |
| Address | 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan |
| t.ktmt@hama-med.ac.jp | |
| Phone | +81-53-435-2987 |
| Submit Date | 2021-10-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-11-27 |
| Release Version | 1 |
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Combined analysis:
| Analysis ID | AN003193 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Thermo Acclaim 120 C18 (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | normalized area values |
Chromatography:
| Chromatography ID: | CH002361 |
| Chromatography Summary: | We used a set of linear gradients starting at 20% of solvent B and increasing linearly to 100% over 50 min, maintained at 100% solvent B for 60 min, then decreased linearly to 20% B from 60 min to 60.1 min, and finished with 20% solvent B for the last 10 min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Acclaim 120 C18 (150 x 2.1mm,3um) |
| Column Temperature: | 50 °C |
| Flow Rate: | 300 μL/min |
| Solvent A: | 50% water/25% methanol/25% acetonitrile; 0.1% formic acid; 5 mM ammonium formate |
| Solvent B: | 90% acetonitrile/ 10% water; 0.1% formic acid; 5 mM ammonium formate |
| Chromatography Type: | Reversed phase |
