Summary of Study ST001958

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001245. The data can be accessed directly via it's Project DOI: 10.21228/M8S985 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001958
Study Title	Data on changes in lipid profiles during differentiation and maturation of human subcutaneous white adipocytes analyzed using chromatographic and bioinformatics tools
Study Summary	Three cell lines of Caucasian-derived subcutaneous preadipocytes were divided into five stages (stage-1 to stage-5) from subcutaneous preadipocytes to mature subcutaneous adipocytes filled with many lipid droplets. Lipids were extracted from cells in each stage and processed using untargeted liquid chromatography and Q-Exactive Orbitrap tandem mass spectrometry. The lipids were identified using LipidSearch 4.2.13.
Institute	Hamamatsu University School of Medicine
Last Name	Kitamoto
First Name	Takuya
Address	1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan
Email	t.ktmt@hama-med.ac.jp
Phone	+81-53-435-2987
Submit Date	2021-10-25
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2021-11-27
Release Version	1

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Treatment:

Treatment ID:	TR002050
Treatment Summary:	Preadipocytes were grown in Promocell Preadipocyte Growth Medium until 100% confluent growth was observed (stage-1). Further, their differentiation into adipocytes was induced using Promocell Preadipocyte Differentiation Medium for 3 consecutive days. The post-differentiation adipocytes were designated as stage-2. After induction of differentiation, Promocell Preadipocyte Differentiation Medium was replaced with Promocell Adipocyte Nutrition Medium. The culture was continued for 7 days until the cytoplasm was filled with many lipid droplets, and the old medium was replaced with fresh Promocell Adipocyte Nutrition Medium after every 2 to 3 days during culturing. During this culture period, small lipid droplets began to appear in the cytoplasm (stage -3), followed by more lipid droplets (stage-4) and the cytoplasm filled with many lipid droplets (stage-5).

Treatment ID:

TR002050

Treatment Summary:

Preadipocytes were grown in Promocell Preadipocyte Growth Medium until 100% confluent growth was observed (stage-1). Further, their differentiation into adipocytes was induced using Promocell Preadipocyte Differentiation Medium for 3 consecutive days. The post-differentiation adipocytes were designated as stage-2. After induction of differentiation, Promocell Preadipocyte Differentiation Medium was replaced with Promocell Adipocyte Nutrition Medium. The culture was continued for 7 days until the cytoplasm was filled with many lipid droplets, and the old medium was replaced with fresh Promocell Adipocyte Nutrition Medium after every 2 to 3 days during culturing. During this culture period, small lipid droplets began to appear in the cytoplasm (stage -3), followed by more lipid droplets (stage-4) and the cytoplasm filled with many lipid droplets (stage-5).