Summary of Study ST001958
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001245. The data can be accessed directly via it's Project DOI: 10.21228/M8S985 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001958 |
| Study Title | Data on changes in lipid profiles during differentiation and maturation of human subcutaneous white adipocytes analyzed using chromatographic and bioinformatics tools |
| Study Summary | Three cell lines of Caucasian-derived subcutaneous preadipocytes were divided into five stages (stage-1 to stage-5) from subcutaneous preadipocytes to mature subcutaneous adipocytes filled with many lipid droplets. Lipids were extracted from cells in each stage and processed using untargeted liquid chromatography and Q-Exactive Orbitrap tandem mass spectrometry. The lipids were identified using LipidSearch 4.2.13. |
| Institute | Hamamatsu University School of Medicine |
| Last Name | Kitamoto |
| First Name | Takuya |
| Address | 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan |
| t.ktmt@hama-med.ac.jp | |
| Phone | +81-53-435-2987 |
| Submit Date | 2021-10-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-11-27 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002044 |
| Sampleprep Summary: | The cell suspension was transferred to a tube and centrifuged at 220g for 3 min, the supernatant was discarded and the cells were suspended in 100 μL of Milli-Q water. Each sample was normalized by measuring the amount of protein by bicinchoninic acid (BCA) protein assay using Pierce Micro BCA Protein Assay Kit (Pierce, Rockford, IL, USA) and the final volume of 600 μL was adjusted using water.10 μL of Splash Lipidomix internal standards (Avanti, Alabaster, AL, USA) was added and the Bligh and Dyer method was used for lipid extraction. |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | -20℃ |
