Summary of Study ST000612
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000448. The data can be accessed directly via it's Project DOI: 10.21228/M84W37 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000612 |
| Study Title | Sphingolipid Analysis of Human Aqueous Humor in Glaucomatous and Control eyes (part I) |
| Study Summary | This study examined the profiles of sphin¬golipids and ceramides present in the aqueous humor (AH) of human control and POAG donors. Furthermore, we quantitatively compared distinct differences between glaucomatous and age-matched control eyes, identifying potential molecules for further experimentation to determine their biological role in modulating cell behavior. Lipids were identified and ratiometrically quantified in a two-step process using precursor ion scan (PIS) or neutral loss scan (NLS) with appropriate class-specific lipid standards on a TSQ Quantum Access Max mass spectrometer following established procedures. We identified several species of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramides that were common between control and glaucomatous AQH. Some unique lipid species in these classes were also identified in controls but not in glaucoma and vice versa. |
| Institute | University of Miami |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | 1638 NW 10th Avenue, Miami, Florida, 33136, USA |
| sbhattacharya@med.miami.edu | |
| Phone | 3054824103 |
| Submit Date | 2017-05-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2019-07-17 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO000629 |
| Collection Summary: | Aqueous humor from POAG and control human subjects (n=20 each totaling 40 samples) were procured following institutional review board approved protocols and adhering to the tenets of the Declaration of Helsinki. AH samples were stored at −80 °C until time of use. The mean age of donors was 71.2 +/- 10.2 and both genders were included in these studies. |
| Sample Type: | Eye tissue |
