Summary of Study ST000612
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000448. The data can be accessed directly via it's Project DOI: 10.21228/M84W37 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000612 |
Study Title | Sphingolipid Analysis of Human Aqueous Humor in Glaucomatous and Control eyes (part I) |
Study Summary | This study examined the profiles of sphin¬golipids and ceramides present in the aqueous humor (AH) of human control and POAG donors. Furthermore, we quantitatively compared distinct differences between glaucomatous and age-matched control eyes, identifying potential molecules for further experimentation to determine their biological role in modulating cell behavior. Lipids were identified and ratiometrically quantified in a two-step process using precursor ion scan (PIS) or neutral loss scan (NLS) with appropriate class-specific lipid standards on a TSQ Quantum Access Max mass spectrometer following established procedures. We identified several species of sphingomyelin, sphingoid base, sphingoid base-1-phosphate, and ceramides that were common between control and glaucomatous AQH. Some unique lipid species in these classes were also identified in controls but not in glaucoma and vice versa. |
Institute | University of Miami |
Last Name | Bhattacharya |
First Name | Sanjoy |
Address | 1638 NW 10th Avenue, Miami, Florida, 33136, USA |
sbhattacharya@med.miami.edu | |
Phone | 3054824103 |
Submit Date | 2017-05-26 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | MS(Dir. Inf.) |
Release Date | 2019-07-17 |
Release Version | 1 |
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Project:
Project ID: | PR000448 |
Project DOI: | doi: 10.21228/M84W37 |
Project Title: | Sphingolipid Profiling of Human Aqueous Humor and Trabecular Meshwork in Glaucomatous and Control eyes |
Project Summary: | Using a triple quadrupole mass spectrometer and direct infusion lipidomics we identified various sphingolipids present in both the aqueous humor and trabecular meshwork of the human eye and compared them between glaucomatous and control groups. |
Institute: | University of Miami |
Last Name: | Bhattacharya |
First Name: | Sanjoy |
Address: | 1638 NW 10th Avenue, Miami, Florida, 33136, USA |
Email: | sbhattacharya@med.miami.edu |
Phone: | 3054824103 |