Summary of Study ST002148
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001361. The data can be accessed directly via it's Project DOI: 10.21228/M8SQ6J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002148 |
| Study Title | Untargeted MS-based metabolomics analysis of the responses to drought stress in Quercus ilex leaf seedlings, and identification of putative compounds related to tolerance |
| Study Type | Untargeted MS-based metabolomics |
| Study Summary | The effect and responses to drought stress have been analyzed in Quercus ilex seedlings by using a non-targeted metabolomic approach, implementing previous studies pub-lished in which other -omics platforms, transcriptomics, and proteomics, have been em-ployed. This work is aimed at characterizing the Q. ilex leaf metabolome, determining possible mechanisms and molecular markers of drought tolerance, and identifying puta-tive bioactive compounds. Six-month-old seedling leaves, subjected to drought stress im-posed by water withholding under high temperature and irradiance conditions, were col-lected when leaf fluorescence decreased by 20 % (day 17) and 45 % (day 24) relative to ir-rigated seedlings. A total of 3934 compounds were resolved, with 616 being variable, and 342 identified, belonging to five chemical families. Out of the identified compounds 33 were variable, mostly corresponding to amino acids, carboxylic acids, benzenoids, flavo-noids and isoprenoids. Epigallocatechin, ellagic acid, pulegone, indole-3-acrylic acid and dihydrozeatin-O-glucoside were up-accumulated under drought conditions at both sam-pling times. An integrated multi-omics analysis of phenolic compounds and related en-zymes was performed, revealing that some enzymes involved in the flavonoid pathways (chalcone synthase, anthocyanidin synthase and anthocyanidin reductase) were up-accumulated at day 24 in non-irrigated seedlings. Some putative markers of drought tol-erance to drought in Q. ilex are proposed for assisting breeding programs based on the se-lection of elite genotypes. |
| Institute | Universidad de Córdoba |
| Department | Department Biochemistry and Molecular Biology |
| Laboratory | Agroforestry and Plant Biochemistry, Proteomics and Systems Biology |
| Last Name | López Hidalgo |
| First Name | Cristina |
| Address | Campus de Rabanales; Edificio C6, Planta Baja |
| lopezhcristina@uniovi.es | |
| Phone | 626894948 |
| Submit Date | 2022-02-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-05-09 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002227 |
| Collection Summary: | Out of the biological replicates, three asymptomatic (non-damaged) non-irrigated seedlings were randomly selected for metabolomics. All leaves were collected when the chlorophyll fluorescence dropped by 20 and 45 % in the non-irrigated seedlings compared to irrigated ones (at days 17 and 24, respectively) [7]. After collection, leaves from the three biological replicates per treatment and sampling time were washed with tap water, blot dried with filter paper, shock-frozen in liquid nitrogen, and stored at -80 °C until metabolite extraction. |
| Sample Type: | new |
| Storage Conditions: | -80℃ |
