Summary of Study ST002148

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001361. The data can be accessed directly via it's Project DOI: 10.21228/M8SQ6J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002148
Study TitleUntargeted MS-based metabolomics analysis of the responses to drought stress in Quercus ilex leaf seedlings, and identification of putative compounds related to tolerance
Study TypeUntargeted MS-based metabolomics
Study SummaryThe effect and responses to drought stress have been analyzed in Quercus ilex seedlings by using a non-targeted metabolomic approach, implementing previous studies pub-lished in which other -omics platforms, transcriptomics, and proteomics, have been em-ployed. This work is aimed at characterizing the Q. ilex leaf metabolome, determining possible mechanisms and molecular markers of drought tolerance, and identifying puta-tive bioactive compounds. Six-month-old seedling leaves, subjected to drought stress im-posed by water withholding under high temperature and irradiance conditions, were col-lected when leaf fluorescence decreased by 20 % (day 17) and 45 % (day 24) relative to ir-rigated seedlings. A total of 3934 compounds were resolved, with 616 being variable, and 342 identified, belonging to five chemical families. Out of the identified compounds 33 were variable, mostly corresponding to amino acids, carboxylic acids, benzenoids, flavo-noids and isoprenoids. Epigallocatechin, ellagic acid, pulegone, indole-3-acrylic acid and dihydrozeatin-O-glucoside were up-accumulated under drought conditions at both sam-pling times. An integrated multi-omics analysis of phenolic compounds and related en-zymes was performed, revealing that some enzymes involved in the flavonoid pathways (chalcone synthase, anthocyanidin synthase and anthocyanidin reductase) were up-accumulated at day 24 in non-irrigated seedlings. Some putative markers of drought tol-erance to drought in Q. ilex are proposed for assisting breeding programs based on the se-lection of elite genotypes.
Institute
Universidad de Córdoba
DepartmentDepartment Biochemistry and Molecular Biology
LaboratoryAgroforestry and Plant Biochemistry, Proteomics and Systems Biology
Last NameLópez Hidalgo
First NameCristina
AddressCampus de Rabanales; Edificio C6, Planta Baja
Emaillopezhcristina@uniovi.es
Phone626894948
Submit Date2022-02-25
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-05-09
Release Version1
Cristina López Hidalgo Cristina López Hidalgo
https://dx.doi.org/10.21228/M8SQ6J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Quercus ilex (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA205846190218_Blankneg4Blank
SA205847190218_Blankneg3Blank
SA205848190218_Blankneg2Blank
SA205849190218_Blankneg5Blank
SA205850190218_Blankneg7Blank
SA205851190218_Naringina_1ppm_negBlank
SA205852190218_Blankneg8Blank
SA205853190218_Blankneg1Blank
SA205854190218_Blankneg6Blank
SA205855190218_Blankpos1Blank
SA205856190218_Blankpos7Blank
SA205857190218_Blankpos8Blank
SA205858190218_Naringina_1ppm_posBlank
SA205859190218_Blankpos6Blank
SA205860190218_Blankpos5Blank
SA205861190218_Blankpos2Blank
SA205862190218_Blankpos3Blank
SA205863190218_Blankpos4Blank
SA205864190218_2_C_T1_R2_posC_T1
SA205865190218_1_C_T1_R1_negC_T1
SA205866190218_3_C_T1_R3_posC_T1
SA205867190218_1_C_T1_R1_posC_T1
SA205868190218_2_C_T1_R2_negC_T1
SA205869190218_3_C_T1_R3_negC_T1
SA205870190218_6_C_T2_R3_posC_T2
SA205871190218_4_C_T2_R1_posC_T2
SA205872190218_5_C_T2_R2_posC_T2
SA205873190218_6_C_T2_R3_negC_T2
SA205874190218_4_C_T2_R1_negC_T2
SA205875190218_5_C_T2_R2_negC_T2
SA205876190218_7_D_T1_R1_posD_T1
SA205877190218_8_D_T1_R2_posD_T1
SA205878190218_9_D_T1_R3_posD_T1
SA205879190218_9_D_T1_R3_negD_T1
SA205880190218_7_D_T1_R1_negD_T1
SA205881190218_8_D_T1_R2_negD_T1
SA205882190218_11_D_T2_R2_negD_T2
SA205883190218_11_D_T2_R2_posD_T2
SA205884190218_10_D_T2_R1_negD_T2
SA205885190218_12_D_T2_R3_posD_T2
SA205886190218_10_D_T2_R1_posD_T2
SA205887190218_12_D_T2_R3_negD_T2
SA205888190218_QCMIX_pos_FS_5Quality Control mixture
SA205889190218_QCMIX_pos_FS_4Quality Control mixture
SA205890190218_QCMIX_pos_FS_3Quality Control mixture
SA205891190218_QCMIX_pos_FS_6Quality Control mixture
SA205892190218_QCMIX_pos_TOP5_2Quality Control mixture
SA205893190218_QCMIX_neg_FS_4Quality Control mixture
SA205894190218_QCMIX_pos_TOP5_3Quality Control mixture
SA205895190218_QCMIX_pos_FS_2Quality Control mixture
SA205896190218_QCMIX_pos_TOP5_1Quality Control mixture
SA205897190218_QCMIX_neg_FS_5Quality Control mixture
SA205898190218_QCMIX_neg_TOP5_3Quality Control mixture
SA205899190218_QCMIX_neg_FS_2Quality Control mixture
SA205900190218_QCMIX_neg_FS_1Quality Control mixture
SA205901190218_QCMIX_neg_TOP5_2Quality Control mixture
SA205902190218_QCMIX_neg_TOP5_1Quality Control mixture
SA205903190218_QCMIX_neg_FS_3Quality Control mixture
SA205904190218_QCMIX_neg_FS_6Quality Control mixture
SA205905190218_QCMIX_pos_FS_1Quality Control mixture
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