Summary of Study ST002148

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001361. The data can be accessed directly via it's Project DOI: 10.21228/M8SQ6J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002148
Study Title	Untargeted MS-based metabolomics analysis of the responses to drought stress in Quercus ilex leaf seedlings, and identification of putative compounds related to tolerance
Study Type	Untargeted MS-based metabolomics
Study Summary	The effect and responses to drought stress have been analyzed in Quercus ilex seedlings by using a non-targeted metabolomic approach, implementing previous studies pub-lished in which other -omics platforms, transcriptomics, and proteomics, have been em-ployed. This work is aimed at characterizing the Q. ilex leaf metabolome, determining possible mechanisms and molecular markers of drought tolerance, and identifying puta-tive bioactive compounds. Six-month-old seedling leaves, subjected to drought stress im-posed by water withholding under high temperature and irradiance conditions, were col-lected when leaf fluorescence decreased by 20 % (day 17) and 45 % (day 24) relative to ir-rigated seedlings. A total of 3934 compounds were resolved, with 616 being variable, and 342 identified, belonging to five chemical families. Out of the identified compounds 33 were variable, mostly corresponding to amino acids, carboxylic acids, benzenoids, flavo-noids and isoprenoids. Epigallocatechin, ellagic acid, pulegone, indole-3-acrylic acid and dihydrozeatin-O-glucoside were up-accumulated under drought conditions at both sam-pling times. An integrated multi-omics analysis of phenolic compounds and related en-zymes was performed, revealing that some enzymes involved in the flavonoid pathways (chalcone synthase, anthocyanidin synthase and anthocyanidin reductase) were up-accumulated at day 24 in non-irrigated seedlings. Some putative markers of drought tol-erance to drought in Q. ilex are proposed for assisting breeding programs based on the se-lection of elite genotypes.
Institute	Universidad de Córdoba
Department	Department Biochemistry and Molecular Biology
Laboratory	Agroforestry and Plant Biochemistry, Proteomics and Systems Biology
Last Name	López Hidalgo
First Name	Cristina
Address	Campus de Rabanales; Edificio C6, Planta Baja
Email	lopezhcristina@uniovi.es
Phone	626894948
Submit Date	2022-02-25
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2022-05-09
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002240
Sampleprep Summary:	Metabolites were extracted from leaves as described by Valledor et al., [41], with minor modifications. An extraction solution containing 600 µL of ice-cold methanol: chloroform: water (5:2:2) was added to 50 mg dry weight leaf tissue and vortexed for 10 sec. The mixture was sonicated (ultrasonic bath, 40 kHz for 10 min) and after centrifugation at 20,000 × g at 4°C for 4 mins, the supernatant was transferred to a new tube. Then, 200 µL of cold methanol: chloroform: water (5:2:2) was added to the pellets and the process was repeated once. After combining both supernatants, they were vacuum dried at 30°C (Speedvac, Eppendorf Vacuum Concentrator Plus/5301, Eppendorf, Leicestershire, UK). Dried extracts were reconstituted in methanol, centrifuged at 20,000×g for 10 min, and filtered through 0.22 μm PTPE membranes (Thermo Fisher Scientific, Courtaboeuf, France), and the filtrate was collected in 1.5 mL LC/MS certified sample vials.

Sampleprep ID:

SP002240

Sampleprep Summary:

Metabolites were extracted from leaves as described by Valledor et al., [41], with minor modifications. An extraction solution containing 600 µL of ice-cold methanol: chloroform: water (5:2:2) was added to 50 mg dry weight leaf tissue and vortexed for 10 sec. The mixture was sonicated (ultrasonic bath, 40 kHz for 10 min) and after centrifugation at 20,000 × g at 4°C for 4 mins, the supernatant was transferred to a new tube. Then, 200 µL of cold methanol: chloroform: water (5:2:2) was added to the pellets and the process was repeated once. After combining both supernatants, they were vacuum dried at 30°C (Speedvac, Eppendorf Vacuum Concentrator Plus/5301, Eppendorf, Leicestershire, UK). Dried extracts were reconstituted in methanol, centrifuged at 20,000×g for 10 min, and filtered through 0.22 μm PTPE membranes (Thermo Fisher Scientific, Courtaboeuf, France), and the filtrate was collected in 1.5 mL LC/MS certified sample vials.