Summary of Study ST002381
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001531. The data can be accessed directly via it's Project DOI: 10.21228/M8ST4T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002381 |
| Study Title | Ruegeria pomeroyi transporter mutant substrate drawdown |
| Study Summary | The goal of this project was to identify bacterial transporters responsible for uptake of environmentally relevant marine metabolites. We used the model marine heterotrophic bacterium Ruegeria pomeroyi DSS-3, for which an arrayed library of single gene knockout mutants has been generated by selecting isolated from a barcoded transposon mutant library (BasSeq). Knockout mutants of putative transporters were grown on minimal medium with a single substrate as sole carbon source. Mutant defect was assessed by comparing the substrate drawdown of isolated mutants to drawdown by a pooled mutant library (BarSeq), a proxy for wildtype fitness. |
| Institute | University of Georgia |
| Laboratory | Moran Lab, Edison Lab |
| Last Name | Uchimiya |
| First Name | Mario |
| Address | 315 Riverbend Rd, Athens, GA, 30602, USA |
| mario.uchimiya@uga.edu | |
| Phone | (706) 542-8387 |
| Submit Date | 2022-11-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2022-12-27 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002463 |
| Collection Summary: | Cultures, 220 ul in 96 well plate (Fisher), were centrifuged at 2250 xg for 10 minutes to pellet cells. Supernatant, 200 ul, was collected and transferred to new 96 well plate. Samples were stored at -80 oC until further processing. |
| Collection Protocol Filename: | 2_Collection protocol_UGA_mutant_Nov2022.docx |
| Sample Type: | Artificial sea water |
| Storage Conditions: | -80℃ |
