Summary of Study ST002381

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001531. The data can be accessed directly via it's Project DOI: 10.21228/M8ST4T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002381
Study TitleRuegeria pomeroyi transporter mutant substrate drawdown
Study SummaryThe goal of this project was to identify bacterial transporters responsible for uptake of environmentally relevant marine metabolites. We used the model marine heterotrophic bacterium Ruegeria pomeroyi DSS-3, for which an arrayed library of single gene knockout mutants has been generated by selecting isolated from a barcoded transposon mutant library (BasSeq). Knockout mutants of putative transporters were grown on minimal medium with a single substrate as sole carbon source. Mutant defect was assessed by comparing the substrate drawdown of isolated mutants to drawdown by a pooled mutant library (BarSeq), a proxy for wildtype fitness.
Institute
University of Georgia
LaboratoryMoran Lab, Edison Lab
Last NameUchimiya
First NameMario
Address315 Riverbend Rd, Athens, GA, 30602, USA
Emailmario.uchimiya@uga.edu
Phone‭(706) 542-8387‬
Submit Date2022-11-16
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2022-12-27
Release Version1
Mario Uchimiya Mario Uchimiya
https://dx.doi.org/10.21228/M8ST4T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Bacteria; Subject species: Ruegeria pomeroyi (Factor headings shown in green)

mb_sample_id local_sample_id Factor_1 (substrate) Factor_2 (bacteria) Factor_2 (time)
SA2374672533-OH butyrate none T0
SA2374682543-OH butyrate none T0
SA2374692593-OH butyrate none Tfinal
SA2374702603-OH butyrate none Tfinal
SA2374713263-OH butyrate pooled-BarSeq Tfinal
SA2374722633-OH butyrate pooled-BarSeq Tfinal
SA2374732753-OH butyrate pooled-BarSeq Tfinal
SA2374742673-OH butyrate ΔSPO2573 Tfinal
SA2374752793-OH butyrate ΔSPO2573 Tfinal
SA2374763743-OH butyrate ΔSPO2573 Tfinal
SA237547456azelaic acid none T0
SA237548455azelaic acid none T0
SA237549477azelaic acid none Tfinal
SA237550476azelaic acid none Tfinal
SA237551544azelaic acid pooled-BarSeq Tfinal
SA237552543azelaic acid pooled-BarSeq Tfinal
SA237553545azelaic acid pooled-BarSeq Tfinal
SA237554589azelaic acid ΔSPO1514 Tfinal
SA237555588azelaic acid ΔSPO1514 Tfinal
SA237556590azelaic acid ΔSPO1514 Tfinal
SA237477106Azeliac none T0
SA237478105Azeliac none T0
SA237479169Azeliac none Tfinal
SA237480170Azeliac none Tfinal
SA237481120Azeliac pooled-BarSeq Tfinal
SA237482122Azeliac pooled-BarSeq Tfinal
SA237483121Azeliac pooled-BarSeq Tfinal
SA237484133Azeliac ΔSPO1514 Tfinal
SA237485132Azeliac ΔSPO1514 Tfinal
SA237486134Azeliac ΔSPO1514 Tfinal
SA237487302Blank none Tfinal
SA237488502Blank none Tfinal
SA237489261Blank none Tfinal
SA237490273Blank none Tfinal
SA237491501Blank none Tfinal
SA237557448cadaverine none T0
SA237558447cadaverine none T0
SA237559468cadaverine none Tfinal
SA237560469cadaverine none Tfinal
SA237561532cadaverine pooled-BarSeq Tfinal
SA237562531cadaverine pooled-BarSeq Tfinal
SA237563533cadaverine pooled-BarSeq Tfinal
SA237564576cadaverine ΔSPO3469 Tfinal
SA237565577cadaverine ΔSPO3469 Tfinal
SA237566578cadaverine ΔSPO3469 Tfinal
SA237567406carnitine none T0
SA237568405carnitine none T0
SA237569419carnitine none Tfinal
SA237570420carnitine none Tfinal
SA237571312carnitine pooled-BarSeq Tfinal
SA237572311carnitine pooled-BarSeq Tfinal
SA237573310carnitine pooled-BarSeq Tfinal
SA237574380carnitine ΔSPO2995 Tfinal
SA237575381carnitine ΔSPO2995 Tfinal
SA237576379carnitine ΔSPO2995 Tfinal
SA237577382carnitine ΔSPO2996 Tfinal
SA237578383carnitine ΔSPO2996 Tfinal
SA237579384carnitine ΔSPO2996 Tfinal
SA237580453choline none T0
SA237581454choline none T0
SA237492104Choline none T0
SA237493103Choline none T0
SA237582475choline none Tfinal
SA237583474choline none Tfinal
SA237494167Choline none Tfinal
SA237495166Choline none Tfinal
SA237584540choline pooled-BarSeq Tfinal
SA237585541choline pooled-BarSeq Tfinal
SA237586542choline pooled-BarSeq Tfinal
SA237496119Choline pooled-BarSeq Tfinal
SA237497117Choline pooled-BarSeq Tfinal
SA237498118Choline pooled-BarSeq Tfinal
SA237587585choline ΔSPO1087 Tfinal
SA237588586choline ΔSPO1087 Tfinal
SA237589587choline ΔSPO1087 Tfinal
SA237499131Choline ΔSPO1087 Tfinal
SA237500129Choline ΔSPO1087 Tfinal
SA237501130Choline ΔSPO1087 Tfinal
SA237590250citrate none T0
SA237591249citrate none T0
SA237592346citrate none Tfinal
SA237593347citrate none Tfinal
SA237594333citrate none Tfinal
SA237595332citrate none Tfinal
SA237596370citrate none Tfinal
SA237597331citrate none Tfinal
SA237598255citrate none Tfinal
SA237599348citrate none Tfinal
SA237600371citrate none Tfinal
SA237601372citrate none Tfinal
SA237602323citrate pooled-BarSeq Tfinal
SA237603324citrate pooled-BarSeq Tfinal
SA237604322citrate pooled-BarSeq Tfinal
SA237605393citrate ΔSPO0184 Tfinal
SA237606392citrate ΔSPO0184 Tfinal
SA237607391citrate ΔSPO0184 Tfinal
SA237608439cysteate none T0
SA237609440cysteate none T0
SA237610462cysteate none Tfinal
SA237611461cysteate none Tfinal
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