Summary of Study ST002381
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001531. The data can be accessed directly via it's Project DOI: 10.21228/M8ST4T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002381 |
Study Title | Ruegeria pomeroyi transporter mutant substrate drawdown |
Study Summary | The goal of this project was to identify bacterial transporters responsible for uptake of environmentally relevant marine metabolites. We used the model marine heterotrophic bacterium Ruegeria pomeroyi DSS-3, for which an arrayed library of single gene knockout mutants has been generated by selecting isolated from a barcoded transposon mutant library (BasSeq). Knockout mutants of putative transporters were grown on minimal medium with a single substrate as sole carbon source. Mutant defect was assessed by comparing the substrate drawdown of isolated mutants to drawdown by a pooled mutant library (BarSeq), a proxy for wildtype fitness. |
Institute | University of Georgia |
Laboratory | Moran Lab, Edison Lab |
Last Name | Uchimiya |
First Name | Mario |
Address | 315 Riverbend Rd, Athens, GA, 30602, USA |
mario.uchimiya@uga.edu | |
Phone | (706) 542-8387 |
Submit Date | 2022-11-16 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2022-12-27 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002476 |
Sampleprep Summary: | Samples were thawed on ice. Each sample, 180 ul, was transferred to a 1.5 ml microcentrifuge tube and mixed with 20 µL of a deuterated phosphate buffer (30 mmol L-1, pH 7.4) and an internal standard 2,2-dimethyl-2-silapentane-5-sulfonate-d6 (DSS, 1 mmol L-1) and transferred to a 3-mm NMR tube (Bruker). |
Sampleprep Protocol Filename: | 4_Sample preparation protocol_UGA_mutant_Nov2022.docx |