Summary of Study ST002383
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001533. The data can be accessed directly via it's Project DOI: 10.21228/M8J99C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002383 |
| Study Title | Metabolomics of B-cell Acute Lymphoblastic Leukemia in Response to Adipocyte Conditioned Media |
| Study Type | Untargeted MS |
| Study Summary | Adipocyte conditioned media (ACM), stromal cell conditioned media (SCM) and unconditioned media (UCM) were added to B-cell Acute Lymphoblastic Leukemia cells (REH and RCH-AcV) either with or without methotrexate (MTX). The metabolomic profiles of the cells was determined by mass spectrometry. |
| Institute | Emory University |
| Department | Pediatrics |
| Laboratory | Joshua Chandler, PhD |
| Last Name | Chandler |
| First Name | Joshua |
| Address | 2015 Uppergate Drive NE, Atlanta, GA 30322 |
| joshua.chandler@emory.edu | |
| Phone | 404-727-3536 |
| Submit Date | 2022-10-07 |
| Num Groups | Two sets of two sets of three groups (MTX and no-MTX, REH and RCH-AcV, ACM vs SCM vs UCM) |
| Total Subjects | 3 replicates of each group (36 total) |
| Publications | submitted to JNCI |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-06-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO002465 |
| Collection Summary: | Human B-ALL cell lines (REH and RCH-AcV) were cultured for 24 hours in UCM, SCM, or ACM. Additionally, MTX-treated human B-ALL cells were cultured for an additional 12 hours of treatment with MTX (50nM for REH and 70 nM for RCH-AcV). This timepoint was chosen due to our prior work in this system to avoid submitting mostly apoptotic cells for analysis [18]. After the prescribed time, leukemia cells were harvested for analysis. Notably, cell viability at the time of harvest exceeded 85% in each culture (n=3 biological replicates). Cell viability was measured using cell a Bio-Rad cell counter (cat#TC20, Bio-Rad, Hercules, CA) and over 1 million viable cells were collected and washed with 1X cold PBS (pH7.4). Cell pellets were snap-frozen in dry ice and stored at -80oC until samples were submitted to our core facility. |
| Sample Type: | B cells |
| Storage Conditions: | -80℃ |
