Summary of Study ST002383
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001533. The data can be accessed directly via it's Project DOI: 10.21228/M8J99C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002383 |
Study Title | Metabolomics of B-cell Acute Lymphoblastic Leukemia in Response to Adipocyte Conditioned Media |
Study Type | Untargeted MS |
Study Summary | Adipocyte conditioned media (ACM), stromal cell conditioned media (SCM) and unconditioned media (UCM) were added to B-cell Acute Lymphoblastic Leukemia cells (REH and RCH-AcV) either with or without methotrexate (MTX). The metabolomic profiles of the cells was determined by mass spectrometry. |
Institute | Emory University |
Department | Pediatrics |
Laboratory | Joshua Chandler, PhD |
Last Name | Chandler |
First Name | Joshua |
Address | 2015 Uppergate Drive NE, Atlanta, GA 30322 |
joshua.chandler@emory.edu | |
Phone | 404-727-3536 |
Submit Date | 2022-10-07 |
Num Groups | Two sets of two sets of three groups (MTX and no-MTX, REH and RCH-AcV, ACM vs SCM vs UCM) |
Total Subjects | 3 replicates of each group (36 total) |
Publications | submitted to JNCI |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-06-07 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002478 |
Sampleprep Summary: | Cell samples (1x106) were mixed with 300 µl of ice-cold 2:1 acetonitrile:water and vortexed. All samples were incubated 30 min on ice and centrifuged at 20,627 g for 10 min at 4 ˚C. The supernatant was transferred to autosampler vials (ThermoFisher) held at 4 ˚C for LC-MS acquisition. |
Processing Storage Conditions: | On ice |