Summary of Study ST002114
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001192. The data can be accessed directly via it's Project DOI: 10.21228/M8MX3X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002114 |
Study Title | Involvement of Mieap in Cardiolipin metabolism (part I-revised) |
Study Summary | Cardiolipin (CL) alterations cause mitochondrial dysfunction. Mieap is involved in mitochondrial quality control (MQC). To investigate whether Mieap functions in MQC via regulation of CL metabolism, quantitative assessment of total CL and comparison of CL species conducted with A549 (Ad-Mieap infected vs. non-infected) and LS174T cells (LS174T-cont vs. Mieap-KD). The A549 cells were harvested 24 hr after infection with Ad-Mieap and were compared with non-infected cells by mass spectrometric analysis. The LS174T-cont and Mieap-KD cells incubated under a normal condition were harvested and subjected to mass spectrometric analysis. |
Institute | National Cancer Center Japan Research Institute |
Last Name | Ikari |
First Name | Naoki |
Address | 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan |
nikari@ncc.go.jp | |
Phone | +81335422511 |
Submit Date | 2022-02-13 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf, raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-03-21 |
Release Version | 2 |
Release Comments | Replacement for ST001893 (datatrack 2779) |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003461 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | UltiMate 3000 (Thermo Fisher Scientific) |
Column | Waters X-Bridge C18 (150 x 1.0mm,3.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | NEGATIVE |
Units | pmol/10E6 cells |
MS:
MS ID: | MS003223 |
Analysis ID: | AN003461 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | pmol/10,000,000 cells |
Ion Mode: | NEGATIVE |