Summary of Study ST002114
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001192. The data can be accessed directly via it's Project DOI: 10.21228/M8MX3X This work is supported by NIH grant, U2C- DK119886.
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| Study ID | ST002114 |
| Study Title | Involvement of Mieap in Cardiolipin metabolism (part I-revised) |
| Study Summary | Cardiolipin (CL) alterations cause mitochondrial dysfunction. Mieap is involved in mitochondrial quality control (MQC). To investigate whether Mieap functions in MQC via regulation of CL metabolism, quantitative assessment of total CL and comparison of CL species conducted with A549 (Ad-Mieap infected vs. non-infected) and LS174T cells (LS174T-cont vs. Mieap-KD). The A549 cells were harvested 24 hr after infection with Ad-Mieap and were compared with non-infected cells by mass spectrometric analysis. The LS174T-cont and Mieap-KD cells incubated under a normal condition were harvested and subjected to mass spectrometric analysis. |
| Institute | National Cancer Center Japan Research Institute |
| Last Name | Ikari |
| First Name | Naoki |
| Address | 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan |
| nikari@ncc.go.jp | |
| Phone | +81335422511 |
| Submit Date | 2022-02-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-03-21 |
| Release Version | 2 |
| Release Comments | Replacement for ST001893 (datatrack 2779) |
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Treatment:
| Treatment ID: | TR002211 |
| Treatment Summary: | Infection of the A549 cell line was carried out by adding viral solution (Ad-Mieap) to A549 cell monolayers, and incubating at 37°C for 120 min with brief agitation every 20 min. This was followed by the addition of culture medium and the return of the infected cells to the 37°C incubator. We established a Mieap-KD cell line using LS174T. Mieap expression was inhibited in the cell line by retroviral expression of short-hairpin RNA (shRNA) against the Mieap sequence. We also established LS174T-cont cells using the retroviral vector with target sequence for EGFP. The LS174T-cont and Mieap-KD cells were incubated under normal condition. |
| Treatment Doseduration: | A549 cells: 24 h; LS174T-cont and Mieap-KD cells: none (incubated under normal condition) |
| Treatment Vehicle: | A549 cells: viral solution (Ad-Mieap); LS174T-cont and Mieap-KD cells: none (incubated under normal condition) |
| Cell Storage: | stored at -80°C |
