Summary of Study ST000366
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000291. The data can be accessed directly via it's Project DOI: 10.21228/M8F600 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000366 |
| Study Title | Metabolomics analysis of colon adenoma in African Americans |
| Study Type | Metabolomics |
| Study Summary | This NMR Metabolomics analysis was performed on feces samples derived from healthy (n = 10) and adenoma (n = 10) African American subjects with the goal of identifying perturbations in metabolomics profiles in colon cancer. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Num Groups | 2 |
| Total Subjects | 20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2017-07-10 |
| Release Version | 1 |
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Project:
| Project ID: | PR000291 |
| Project DOI: | doi: 10.21228/M8F600 |
| Project Title: | Microbiomic analysis in African American with Colonic Lesions |
| Project Type: | Microbiome |
| Project Summary: | Increasing evidence suggests a role in colorectal carcinogenesis to the gut microbiota. However, no specific bacteria have been unequivocally linked to either initiation or progression of colorectal cancer (CRC). A Microbiome analysis was conducted to analyze the microbiota composition and functional potential in African Americans with colorectal lesions with the goal of detecting markers of diagnostic value. DNA from 10 CRC tumors and their matched normal tissues as well as stool samples from 10 colon adenomas and 10 healthy subjects were analyzed for their bacterial composition and genomic content. 16S rDNA amplicons were analyzed by HITChip 16S microarray and by sequencing in stool samples and colon tissues, respectively. The functional potential was determined by sequence-based metagenomics using Illumina at a depth of 15 million reads per tissue sample to compensate for the host’s DNA presence. For the stools, the metagenomic sequencing was performed at 3 million reads per sample. Metagenomic Linkage Groups (MLGs) were established and those with high discriminative power between healthy and neoplastic specimens were analyzed for their genetic content. Also, metagenomic reads from stool samples were mapped against bacterial genes from tissues and reads from tissues were mapped against stools assembled bacterial genes to identify common markers with discriminative power. |
| Institute: | Howard University |
| Department: | Department of Pathology and Cancer Center |
| Last Name: | Brim |
| First Name: | Hassan |
| Address: | 2041 Georgia Avenue, N.W., Washington, DC 20060 |
| Email: | hbrim@howard.edu |
| Phone: | 202-806-4198 |
| Funding Source: | NIH |
