Summary of Study ST000366

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000291. The data can be accessed directly via it's Project DOI: 10.21228/M8F600 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000366
Study Title	Metabolomics analysis of colon adenoma in African Americans
Study Type	Metabolomics
Study Summary	This NMR Metabolomics analysis was performed on feces samples derived from healthy (n = 10) and adenoma (n = 10) African American subjects with the goal of identifying perturbations in metabolomics profiles in colon cancer.
Institute	University of North Carolina
Department	Systems and Translational Sciences
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Num Groups	2
Total Subjects	20
Raw Data Available	Yes
Raw Data File Type(s)	fid
Analysis Type Detail	NMR
Release Date	2017-07-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000394
Sampleprep Summary:	Fecal samples (20 mg) were mixed with 1000 μL of D2O, mixed thoroughly by vortexing, and then centrifuged at 16000 x g for 20 min. The supernatants were removed and filtered through 0.22μ centrifuge filters at 16000 x g for 20 minutes. 540 μl of the filtrate was mixed with 60 μl of Chenomix Internal Standard mixture (containing DSS, Imidazole, and NaN3 in D2O). Aliquots of 550 μl were then transferred into 5mm NMR tubes.

Sampleprep ID:

SP000394

Sampleprep Summary:

Fecal samples (20 mg) were mixed with 1000 μL of D2O, mixed thoroughly by vortexing, and then centrifuged at 16000 x g for 20 min. The supernatants were removed and filtered through 0.22μ centrifuge filters at 16000 x g for 20 minutes. 540 μl of the filtrate was mixed with 60 μl of Chenomix Internal Standard mixture (containing DSS, Imidazole, and NaN3 in D2O). Aliquots of 550 μl were then transferred into 5mm NMR tubes.