Summary of study ST000166

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000144. The data can be accessed directly via it's Project DOI: 10.21228/M8C01P This work is supported by NIH grant, U2C- DK119886.

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Study IDST000166
Study TitleCardiac Resynchronization Therapy Induces Adaptive Metabolic Transitions in the Metabolomic Profile of Heart Failure
Study Typeintervention
Study SummaryThis prospective study consisted of 24 patients undergoing CRT for advanced HF and 10 control patients who underwent catheter ablation for supraventricular arrhythmia but not CRT. Blood samples were collected before and 3 months after CRT. Metabolomic profiling of plasma samples was performed using gas chromatography–mass spectrometry and nuclear magnetic resonance.
Institute
Mayo Clinic
DepartmentDepartment of Medicine
Last NameCha
First NameYong-Mei
Emailycha@mayo.edu
Submit Date2015-05-14
Num Groups3
Total Subjects24
Raw Data AvailableNo
Analysis Type DetailGC-MS
Release Date2015-06-28
Release Version1
Yong-Mei Cha Yong-Mei Cha
https://dx.doi.org/10.21228/M8C01P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000185
Sampleprep Summary:For gas chromatography–mass spectrometry (GC-MS), plasma (100 ?L) was extracted using a 900-?L methanol:water (8:1, v/v) mixture containing 5 ?g internal standard, myristic-d27 acid, at ambient temperature 33. Supernatant (900 ?L) was transferred and completely dried in a vacuum concentrator. Subsequently, the tubes were methoximated and derivatized and then analyzed using an Agilent 6890 GC oven with Agilent 5973 MS 34.
Sample Treatment and Instrumental Conditions for 1H NMR Metabolomic Analysis
For nuclear magnetic resonance (NMR) imaging, plasma (60 ?L) was diluted 140 ?L with 0.2M phosphate buffer (pH 7.4):D2O containing a mixture of 16 mM formate and 4 mM TSP (1:1, v/v). After filtering (0.22 ?m), the samples were transferred into a 3-mm-diameter NMR tube. High-resolution 1H NMR spectra were acquired at 600 MHz on a Bruker Avance III 600 spectrometer. Spectra peaks were identified according to Chenomx NMR Suite 6.1 software and data in the literature 35, 36.
Sampleprep Protocol Filename:Methods-For-Upload.docx
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