Summary of Study ST001664
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001069. The data can be accessed directly via it's Project DOI: 10.21228/M8HQ3X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001664 |
| Study Title | E.coli K-12 treated by IPL - analysis of polar phase (part-II) |
| Study Summary | E.coli K-12 cells were treated by IPL, extracted and separated into organic/lipid phase and polar phase. Chemical derivatization with dansyl chloride was applied for analysis of amino acids in the polar phase extraction. |
| Institute | University of Minnesota |
| Last Name | Chen |
| First Name | Chi |
| Address | 1334 Eckles Ave, St Paul, Minnesota, 55108, USA |
| chichen@umn.edu | |
| Phone | 6126247704 |
| Submit Date | 2021-01-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-02-17 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001747 |
| Sampleprep Summary: | The control and IPL-treated E. coli samples were centrifuged for obtaining the pellets, which were then washed with PBS twice. After extraction with methanol-water-chloroform, the polar phase was separated and derivatized with dansyl chloride (DC) for amino acid analysis. |
| Sampleprep Protocol Filename: | Cell_culture_IPL treatment_Sample preparation_method.docx |
