Summary of Study ST001664

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001069. The data can be accessed directly via it's Project DOI: 10.21228/M8HQ3X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001664
Study TitleE.coli K-12 treated by IPL - analysis of polar phase (part-II)
Study SummaryE.coli K-12 cells were treated by IPL, extracted and separated into organic/lipid phase and polar phase. Chemical derivatization with dansyl chloride was applied for analysis of amino acids in the polar phase extraction.
Institute
University of Minnesota
Last NameChen
First NameChi
Address1334 Eckles Ave, St Paul, Minnesota, 55108, USA
Emailchichen@umn.edu
Phone6126247704
Submit Date2021-01-25
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2021-02-17
Release Version1
Chi Chen Chi Chen
https://dx.doi.org/10.21228/M8HQ3X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001754
Treatment Summary:For each IPL treatment, a 30 mL K-12 suspension in PBS was loaded in a petri dish (15 cm diameter) and then placed under the broad-spectrum light (wavelength 190-1100 nm) in a Z-1000 SteriPulse-XL system (Xenon Corporation, Woburn, MA). The samples were treated with the IPL for 0, 5, 10, 15, and 20 s.
Treatment Protocol Filename:Cell_culture_IPL_treatment_Sample_preparation_method.docx
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