Summary of Study ST001664
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001069. The data can be accessed directly via it's Project DOI: 10.21228/M8HQ3X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001664 |
| Study Title | E.coli K-12 treated by IPL - analysis of polar phase (part-II) |
| Study Summary | E.coli K-12 cells were treated by IPL, extracted and separated into organic/lipid phase and polar phase. Chemical derivatization with dansyl chloride was applied for analysis of amino acids in the polar phase extraction. |
| Institute | University of Minnesota |
| Last Name | Chen |
| First Name | Chi |
| Address | 1334 Eckles Ave, St Paul, Minnesota, 55108, USA |
| chichen@umn.edu | |
| Phone | 6126247704 |
| Submit Date | 2021-01-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-02-17 |
| Release Version | 1 |
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Project:
| Project ID: | PR001069 |
| Project DOI: | doi: 10.21228/M8HQ3X |
| Project Title: | Effect of IPL on E.coli Metabolome hydrophilic phase |
| Project Type: | Untargeted LC-MS metabolomic study |
| Project Summary: | Intense pulsed light (IPL) is becoming a new technical platform for disinfecting food against pathogenic bacteria. Metabolic changes are deemed to occur in bacteria as either the causes or the consequences of IPL-elicited bactericidal and bacteriostatic effects. However, little is known about the influences of IPL on bacterial metabolome. In this study, the IPL treatment was applied to E. coli K-12 for 0-20s, leading to time- and dose-dependent reductions in colony-forming units (CFU) and morphological changes. Cytoplasmic metabolites of the control and IPL-treated E. coli were examined by the liquid chromatography-mass spectrom-etry (LC-MS)-based metabolomic fingerprinting. The results from multivariate modeling and marker identification indicated that the metabolites in redox response, glycolysis, amino acid and nucleotide metabolism were selectively affected by the IPL treatments. |
| Institute: | University of Minnesota |
| Last Name: | Chen |
| First Name: | Chi |
| Address: | 1334 Eckles Ave, St Paul, Minnesota, 55108, USA |
| Email: | chichen@umn.edu |
| Phone: | 6126247704 |
