Summary of Study ST001839
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001161. The data can be accessed directly via it's Project DOI: 10.21228/M8N11H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001839 |
Study Title | Metabolite profiling of Malaysian Gracilaria edulis reveals Eplerenone as novel antibacterial compound for drug repurposing against MDR Bacteria |
Study Type | In vitro antibacterial studies |
Study Summary | The current study re-defines a method to reveal bioactive compounds from the crude extracts of Malaysian red seaweed Gracilaria edulis, having promising antibacterial activities against selected bacterial species. Three species of Gram-positive and - negative characters were remarkably inhibited by the sequential and direct extracts of ethyl acetate and acetone. These were further separated through chromatographic methods to reveal a plethora of chemical constituents to be considered for a downstream virtual screening against selected crucial proteins of the six bacteria. |
Institute | Sunway University |
Department | Biological Sciences, Sunway University, Selangor, Malaysia |
Laboratory | Disease Complexity |
Last Name | Lahiri |
First Name | Chandrajit |
Address | Sunway University, No. 5, Jalan Universiti, Bandar Sunway, Petaling Jaya 47500, Selangor, Malaysia |
chandrajitl@sunway.edu.my | |
Phone | +60 3 7491 8622 |
Submit Date | 2021-04-13 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2021-10-20 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001922 |
Sampleprep Summary: | Essentially, for both approaches, the seaweeds were rinsed sequentially with seawater followed by normal tap and then double distilled water to eradicate dirt and impurities. Clean samples were then dried using a freeze-dryer and later crushed into fine granule powder using an electric grinder. Different fractions of extracts were prepared using 10 grams of each powder to dissolve them in 100 mL of the mentioned solvents. All the prepared mixtures were made homogeneous using a rotating shaker (Yihder LM-530D, Shaker, Taiwan) for 24 hours and finally centrifuged (Eppendorf 5810 R Centrifuge, Germany) at 4000 rpm for 10 min at 4◦C to separate the supernatant. Each of the clear supernatants of the extracts was concentrated via a Rotary evaporator (Thermo Fisher Scientific EYELA N-1200A Rotary Evaporator, Tokyo). A further concentration using a vacuum concentrator (LaboGene, Brigachtal, Germany) was done to obtain a viscous liquid for storage at 4◦C and future experiments. |
Processing Storage Conditions: | Room temperature |
Extract Storage: | 4℃ |