Summary of study ST000257

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000209. The data can be accessed directly via it's Project DOI: 10.21228/M8CP4Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000257
Study TitleNIH WCMC Pilot & Feasibility Project: “Metabolite changes associated with weight loss”
Study TypeFeeding study
Study SummaryTargeted metabolomic analyses of oxylipins, endocannabinoids, and ceramides were performed on 18 mouse liver, adipose, hypothalamus, plasma, and muscle samples collected from mice euthanized at 18 weeks following consumption of three diet regimens that induced lean, obese, and weight loss phenotypes. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization.
Institute
University of California, Davis
DepartmentNutrition
LaboratoryNewman Lab
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Emailjohn.newman@ars.usda.gov
Phone+1-530-752-1009
Submit Date2015-01-16
Num Groups3
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s).mzML
Uploaded File Size128 MB
Analysis Type DetailLC-MS
Release Date2015-09-24
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M8CP4Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000209
Project DOI:doi: 10.21228/M8CP4Q
Project Title:Metabolite changes associated with weight loss
Project Type:Feeding study
Project Summary:This study aims to identify changes in metabolites that occur in metabolic tissues, including liver, adipose (epididymal), hypothalamus, and muscle, as well as plsma, in the obese state that are long-lasting and not reversed by weight loss. Mice were divided into three groups termed LFD (low fat diet), HFD (high fat diet), and SW (switch diet from HFD to LFD)(n = 6 per group). All mice were fed normal chow until 12 weeks of age. The LFD group were fed a low fat diet (10% fat) ad libitum for 18 weeks. The HFD diet group were fed high fat diet (60% fat) ad libitum for 18 weeks. The SW group were fed high fat diet for 9 weeks, then low fat diet for 9 weeks after which their body weights returned to similar levels as the LFD group. Each group was analyzed for metabolite and gene expression changes.
Institute:University of California, San Diego
Department:Department of Medicine
Laboratory:Osborn
Last Name:Osborn
First Name:Olivia
Address:9500 Gilman Dr., La Jolla, CA 92093
Email:oosborn@ucsd.edu
Phone:+1-858-822-6645
Funding Source:National Institute of Health U24 DK092993
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