Summary of Study ST001911
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001205. The data can be accessed directly via it's Project DOI: 10.21228/M8Z700 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001911 |
| Study Title | A high-fat diet induced obesity murine model treated with bitter melon (Momordica charantia) |
| Study Type | A 40-day Intervention experiment |
| Study Summary | Metabolic effects of lyophilized bitter melon juice (BMJ) extract (oral gavage 200mg/kg/body weight-daily for 40 days) intake were evaluated in diet-induced obese C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat]. Changes in metabolite abundance levels in lipid-phase plasma [liquid chromatography mass spectrometry (LC-MS)-based metabolomics] after BMJ intervention were assessed. |
| Institute | University of Colorado Anschutz Medical Campus |
| Department | Pharmaceutical Sciences |
| Laboratory | Dr. Rajesh Agarwal's Laboratory |
| Last Name | Agarwal |
| First Name | Rajesh |
| Address | 12850 E. Montview Blvd, C238, Room V20-2118, Aurora, CO 80045, USA |
| Rajesh.Agarwal@cuanschutz.edu | |
| Phone | 303-724-4055 |
| Submit Date | 2021-05-22 |
| Num Groups | 2 |
| Total Subjects | 8 |
| Num Males | 8 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001205 |
| Project DOI: | doi: 10.21228/M8Z700 |
| Project Title: | Metabolome changes induced by bitter melon (Momordica charantia)- intake in a high-fat diet induced obesity model |
| Project Type: | LCMS quanitative analysis |
| Project Summary: | Quanitative analysis of metabolomics on samples from murine samples (KEGG Pathway MAPS, LIPIDMAPS, Human Metabolome Database) |
| Institute: | University of Colorado Anschutz Medical Campus |
| Department: | Pharmaceutical Sciences |
| Laboratory: | Dr. Rajesh Agarwal's Laboratory and |
| Last Name: | Agarwal |
| First Name: | Rajesh |
| Address: | 12850 E. Montview Blvd, C238, Room V20-2118, Aurora, CO 80045, USA |
| Email: | Rajesh.Agarwal@cuanschutz.edu |
| Phone: | 303-724-4055 |
| Funding Source: | This work was supported by the National Institutes of Health/ National Cancer Institute grant R01CA195708, the National Institutes of Health Office of Dietary Supplements supplemental funds R01CA195708-02S1, and the pilot funding from the office of the Associate Dean for Research and Graduate Education (ADR), School of Pharmacy, UC AMC (to RA), and the National Cancer Institute diversity supplement grant R01CA195708-04S1 (to DR and RA). |
| Contributors: | Dominique Reed, Dileep Kumar, Sushil Kumar, Komal Raina, Reenu Punia, Rama Kant, Laura Saba, Charmion Cruickshank-Quinn, Boris Tabakoff, Nichole Reisdorph, Michael G. Edwards, Michael Wempe, Chapla Agarwal, Rajesh Agarwal |
Subject:
| Subject ID: | SU001989 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wildtype |
| Age Or Age Range: | 4 weeks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory |
| Animal Housing: | UC Denver |
| Animal Feed: | High fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492 |
| Animal Water: | ad libitum |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA176929 | OB_07(raw) | High Fat |
| SA176930 | OB_12_A(raw) | High Fat |
| SA176931 | OB_11(raw) | High Fat |
| SA176932 | OB+BMJ_05(raw) | Treated |
| SA176933 | OB+BMJ_03(raw) | Treated |
| SA176934 | OB+BMJ_04(raw) | Treated |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO001982 |
| Collection Summary: | Blood was collected and plasma/serum flash frozen in liquid nitrogen and stored in -80 degrees Celsius for biochemical/metabolic assessments. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR002001 |
| Treatment Summary: | Sixteen-week-old diet-induced obese (DIO) C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat from Research diets Inc., # D12492) from 4 weeks of age] were purchased from Jackson labs (Bar Harbor, ME). Once received at the animal house facility in UC Denver, the mice were randomized into two groups (n=4/ group): a) Control-DIO group maintained on the 60 kcal% HFD for additional 40 days, and b) BMJ+DIO group maintained on 60 kcal% HFD fat for additional 40 days plus orally gavage with lyophilized bitter melon juice extract [(5mg per mouse; total volume of 100 μl, 200mg/kg body weight/ day), reconstituted in sterile deionized water immediately before use)] throughout the study. |
| Treatment: | Intervention |
| Treatment Compound: | Bitter melon juice |
| Treatment Route: | Oral gavage |
| Treatment Dose: | 200mg/kg body weight/ day |
| Animal Fasting: | On day 40 of study initiation, mice in control and treament groups were subjected to fasting for six hours prior to sacrifice. |
| Animal Endp Euthanasia: | On day 40, mice were sacrificed after CO2 asphyxiation and exsanguination. |
Sample Preparation:
| Sampleprep ID: | SP001995 |
| Sampleprep Summary: | Frozen plasma was stored at -80°C until sample preparation for untargeted liquid-chromatography mass spectrometry (LC-MS)-based metabolomics. Briefly, 100µL of sample underwent protein precipitation using methanol, followed by liquid-liquid extraction using methyl-tert butyl ether as previously described[1, 2] to obtain an aqueous fraction and a lipid fraction. The lipid fraction was dried down and resuspended in 100% methanol for LC-MS analysis. |
| Processing Storage Conditions: | Described in summary |
Combined analysis:
| Analysis ID | AN003108 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Agilent 6210 TOF |
| Ion Mode | POSITIVE |
| Units | Abundance (mass spectral counts) |
Chromatography:
| Chromatography ID: | CH002295 |
| Chromatography Summary: | The samples from the lipid fraction were analyzed an Agilent 1290 series pump with an Agilent Zorbax Rapid Resolution HD (RRHD) SB-C18, 1.8 micron (2.1 x 100 mm) analytical column and an Agilent Zorbax SB-C18, 1.8 micron (2.1 x 5 mm) guard column. The autosampler tray temperature was set at 4°C, column temperature was set at 60°C, and the sample injection volume was 4µL. The flow rate was 0.7 mL/min with the following mobile phases: mobile phase A was water with 0.1% formic acid, and mobile phase B was 60:36:4 isopropyl alcohol:acetonitrile:water with 0.1% formic acid. Gradient elution was as follows: 0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes. |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| Column Temperature: | 60 |
| Flow Gradient: | 0-1 minute 30-70% B, 1-7.92 minutes 70-100% B, 7.92-10.4 minutes 100% B, 10.4-10.5 minutes 100-30% B, followed by column re-equilibration with 30% B from 10.5-15.1 minutes. |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 60% isopropanol/36% acetonitrile/4% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002890 |
| Analysis ID: | AN003108 |
| Instrument Name: | Agilent 6210 TOF |
| Instrument Type: | TOF |
| MS Type: | ESI |
| MS Comments: | The mass spectrometry conditions were as follows: Agilent 6210 Time-of-Flight mass spectrometer (TOF-MS) in positive ionization mode with dual electrospray (ESI) source, mass range 60-1600m/z, scan rate 2.03, gas temperature 300°C, gas flow 12.0L/min, nebulizer 30psi, skimmer 60V, capillary voltage 4000V, fragmentor 120V, reference masses 121.050873 and 922.009798 (Agilent reference mix). Proprietary analytical software for integration and peak picking |
| Ion Mode: | POSITIVE |
