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MGP001623

UniProt Annotations

Entry Information
Gene Nameintegrin-linked kinase
Protein EntryILK_HUMAN
UniProt IDQ13418
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q13418-1; Sequence=Displayed; Name=2; IsoId=Q13418-2; Sequence=VSP_054920; Note=No experimental confirmation available.; Name=3; IsoId=Q13418-3; Sequence=VSP_055925; Note=No experimental confirmation available.;
Catalytic ActivityATP + a protein = ADP + a phosphoprotein.
DomainA PH-like domain is involved in phosphatidylinositol phosphate binding. {ECO:0000269|PubMed:19074270, ECO:0000269|PubMed:19117955}.
Enzyme RegulationStimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K- dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.
FunctionReceptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
InteractionQ14790:CASP8; NbExp=2; IntAct=EBI-747644, EBI-78060; P55211:CASP9; NbExp=2; IntAct=EBI-747644, EBI-516799; Q9H0C8:ILKAP; NbExp=3; IntAct=EBI-747644, EBI-2620298; P48059:LIMS1; NbExp=5; IntAct=EBI-747644, EBI-306928; Q9NVD7:PARVA; NbExp=7; IntAct=EBI-747644, EBI-747655; Q6R327:RICTOR; NbExp=8; IntAct=EBI-747644, EBI-1387196; Q9BWU0:SLC4A1AP; NbExp=8; IntAct=EBI-747644, EBI-1999704; Q9UMS6:SYNPO2; NbExp=6; IntAct=EBI-747644, EBI-3453434;
PtmAutophosphorylated on serine residues. {ECO:0000269|PubMed:21406692}.
Sequence CautionSequence=CAB94832.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact. Was originally thought to be distinct gene (ILK2) (PubMed:10871859).; Evidence={ECO:0000305|PubMed:10871859};
SimilarityBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. {ECO:0000305}.
SimilarityContains 1 protein kinase domain. {ECO:0000255|PROSITE-ProRule:PRU00159}.
SimilarityContains 5 ANK repeats. {ECO:0000255|PROSITE- ProRule:PRU00023}.
Subcellular LocationCell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium {ECO:0000250}. Cytoplasm, myofibril, sarcomere.
SubunitInteracts with FERMT2 (By similarity). Interacts with the cytoplasmic domain of ITGB1. Could also interact with integrin ITGB2, ITGB3 and/or ITGB5. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with PARVA and PARVB; these compete for the same binding site. Interacts probably also with TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. {ECO:0000250, ECO:0000269|PubMed:11402068, ECO:0000269|PubMed:12167643, ECO:0000269|PubMed:15284246, ECO:0000269|PubMed:19074270, ECO:0000269|PubMed:19117955, ECO:0000269|PubMed:19118217, ECO:0000269|PubMed:20005845, ECO:0000269|PubMed:20117114}.
Tissue SpecificityHighly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
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